Ali Kafil, Parisa Mohamadynejad, Mehdi Moghanibashi
{"title":"DNASE1可变数目串联重复序列和单核苷酸多态性与胃癌的显著关联。","authors":"Ali Kafil, Parisa Mohamadynejad, Mehdi Moghanibashi","doi":"10.3389/bjbs.2022.10526","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction:</b> Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in <i>DNASE1</i>, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the <i>DNASE1</i> polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the <i>DNASE1</i> gene with the risk of gastric cancer. <b>Materials and Methods:</b> The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR. <b>Results:</b> The genotype GG (rs1053874) in exon 8 of <i>DNASE1</i> (odds ratio [95% confidence interval]) 4.65 [2.10-10.29], <i>p</i> < 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56-9.01], <i>p</i> = 0.003) are both linked to gastric cancer. <b>Conclusion:</b> We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis markers.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302541/pdf/","citationCount":"0","resultStr":"{\"title\":\"Significant Association of <i>DNASE1</i> Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer.\",\"authors\":\"Ali Kafil, Parisa Mohamadynejad, Mehdi Moghanibashi\",\"doi\":\"10.3389/bjbs.2022.10526\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction:</b> Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in <i>DNASE1</i>, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the <i>DNASE1</i> polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the <i>DNASE1</i> gene with the risk of gastric cancer. <b>Materials and Methods:</b> The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR. <b>Results:</b> The genotype GG (rs1053874) in exon 8 of <i>DNASE1</i> (odds ratio [95% confidence interval]) 4.65 [2.10-10.29], <i>p</i> < 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56-9.01], <i>p</i> = 0.003) are both linked to gastric cancer. <b>Conclusion:</b> We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis markers.</p>\",\"PeriodicalId\":9236,\"journal\":{\"name\":\"British Journal of Biomedical Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2022-05-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302541/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British Journal of Biomedical Science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/bjbs.2022.10526\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Journal of Biomedical Science","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/bjbs.2022.10526","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Significant Association of DNASE1 Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer.
Introduction: Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in DNASE1, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the DNASE1 polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the DNASE1 gene with the risk of gastric cancer. Materials and Methods: The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR. Results: The genotype GG (rs1053874) in exon 8 of DNASE1 (odds ratio [95% confidence interval]) 4.65 [2.10-10.29], p < 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56-9.01], p = 0.003) are both linked to gastric cancer. Conclusion: We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis markers.
期刊介绍:
The British Journal of Biomedical Science is committed to publishing high quality original research that represents a clear advance in the practice of biomedical science, and reviews that summarise recent advances in the field of biomedical science. The overall aim of the Journal is to provide a platform for the dissemination of new and innovative information on the diagnosis and management of disease that is valuable to the practicing laboratory scientist.