sars - covid自组装分裂适体多重检测及孔雀石绿dna适体的小型化

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Sensors and Actuators Reports Pub Date : 2022-11-01 DOI:10.1016/j.snr.2022.100125

Martin R． O'Steen , Dmitry M．Kolpashchikov

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引用次数: 2

摘要

多重分析通常依赖于昂贵的传感器，其中包含共价连接的荧光染料。在此，我们开发了一种基于适配体自组装的多重检测方法。这种多重方法利用先前建立的分裂适体传感器与基于孔雀石绿DNA适体的新型分裂适体传感器结合。该系统能够在一个样品中同时荧光检测两个SARS covid -19相关序列，单个传感器的检测限(LOD)在低nM范围内。对分裂孔雀石绿(SMG)传感器进行优化，得到了一个最小的适体结构，Mini-MG，能够诱导DNA发夹和传感器格式的孔雀石绿荧光。

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A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer

Multiplex assays often rely on expensive sensors incorporating covalently linked fluorescent dyes. Herein, we developed a self-assembling aptamer-based multiplex assay. This multiplex approach utilizes a previously established split aptamer sensor in conjugation with a novel split aptamer sensor based upon a malachite green DNA aptamer. This system was capable of simultaneous fluorescent detection of two SARS COVID-19-related sequences in one sample with individual sensors that possesses a limit of detection (LOD) in the low nM range. Optimization of the Split Malachite Green (SMG) sensor yielded a minimized aptamer construct, Mini-MG, capable of inducing fluorescence of malachite green in both a DNA hairpin and sensor format.

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.