Incorporation of short- and long-chain fatty acids into glycerides by lactating-goat mammary tissue

• 1.

  1. A particulate system obtained from lactating-goat mammary gland catalyzed the incorporation fo [I-¹⁴C]acyl-CoA into glycerides with dl-α-glyceroophosphate or α,β-diglyceride as acyl acceptor.

• 2.

  2. The pathway for the triglyceride synthesis in this system appears to involve the formation of diglyceride form α-glycerophosphate and its subsequent conversion to triglyceride.

• 3.

  3. Oleic, hexanoic, octanoic, and palmitic acids were incorporated into glycerides to different but considerable extents while the incorporation of butyrate was relatively low.

• 4.

  4. The incorporation of palmitate into α-glycerophosphate resulted in the formation of considerable proportion of triglycerides. On the other hand, the incorporation of hexanoate or octanoate into α-glycerophosphate did not proceed beyond the formation of diglycerides to any appreciable extent.

• 5.

  5. Utilization of glycerol for glyceride synthesis was not appreciable in this system.