一种直接高效的体外聚合酶链反应合成哑铃形线性DNA的方法。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.191

Masumi Taki, Yoshio Kato, Makoto Miyagishi, Yasuomi Takagi, Masayuki Sano, Kazunari Taira

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引用次数: 9

摘要

已知含有转录单元的线性、共价闭合、哑铃形DNA载体具有生物稳定性和安全性，有望用于基因治疗。我们建立了一种简单、快速、大规模的制备性合成方法，利用DNA末端的分子内环化来合成修饰和未修饰的哑铃DNA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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A direct and efficient synthesis method for dumbell-shaped linear DNA using PCR in vitro.

A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.

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Nucleic acids research. Supplement (2001)

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