Guoyu Hu, Danqiu Zhang, Dan Luo, Wenhui Sun, Rijin Zhou, Zonglie Hong, Shoaib Munir, Zhibiao Ye, Changxian Yang, Junhong Zhang, Taotao Wang
{"title":"SlTCP24和SlTCP29通过与SlAS2相互作用和激活SlCKX2的转录协同调节番茄复叶发育","authors":"Guoyu Hu, Danqiu Zhang, Dan Luo, Wenhui Sun, Rijin Zhou, Zonglie Hong, Shoaib Munir, Zhibiao Ye, Changxian Yang, Junhong Zhang, Taotao Wang","doi":"10.1111/nph.19221","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n \n </p><ul>\n \n \n <li>The complexity of compound leaves results primarily from the leaflet initiation and arrangement during leaf development. However, the molecular mechanism underlying compound leaf development remains a central research question.</li>\n \n \n <li>SlTCP24 and SlTCP29, two plant-specific transcription factors with the conserved TCP motif, are shown here to synergistically regulate compound leaf development in tomato. When both of them were knocked out simultaneously, the number of leaflets significantly increased, and the shape of the leaves became more complex. SlTCP24 and SlTCP29 could form both homodimers and heterodimers, and such dimerization was impeded by the leaf polarity regulator SlAS2, which interacted with SlTCP24 and SlTCP29.</li>\n \n \n <li>SlTCP24 and SlTCP29 could bind to the TCP-binding <i>cis</i>-element of the <i>SlCKX2</i> promoter and activate its transcription. Transgenic plants with <i>SlTCP24</i> and <i>SlTCP29</i> double-gene knockout had a lowered transcript level of <i>SlCKX2</i> and an elevated level of cytokinin.</li>\n \n \n <li>This work led to the identification of two key regulators of tomato compound leaf development and their targeted genes involved in cytokinin metabolic pathway. A model of regulation of compound leaf development was proposed based on observations of this study.</li>\n </ul>\n \n </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":9.4000,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"SlTCP24 and SlTCP29 synergistically regulate compound leaf development through interacting with SlAS2 and activating transcription of SlCKX2 in tomato\",\"authors\":\"Guoyu Hu, Danqiu Zhang, Dan Luo, Wenhui Sun, Rijin Zhou, Zonglie Hong, Shoaib Munir, Zhibiao Ye, Changxian Yang, Junhong Zhang, Taotao Wang\",\"doi\":\"10.1111/nph.19221\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>\\n \\n </p><ul>\\n \\n \\n <li>The complexity of compound leaves results primarily from the leaflet initiation and arrangement during leaf development. However, the molecular mechanism underlying compound leaf development remains a central research question.</li>\\n \\n \\n <li>SlTCP24 and SlTCP29, two plant-specific transcription factors with the conserved TCP motif, are shown here to synergistically regulate compound leaf development in tomato. When both of them were knocked out simultaneously, the number of leaflets significantly increased, and the shape of the leaves became more complex. SlTCP24 and SlTCP29 could form both homodimers and heterodimers, and such dimerization was impeded by the leaf polarity regulator SlAS2, which interacted with SlTCP24 and SlTCP29.</li>\\n \\n \\n <li>SlTCP24 and SlTCP29 could bind to the TCP-binding <i>cis</i>-element of the <i>SlCKX2</i> promoter and activate its transcription. Transgenic plants with <i>SlTCP24</i> and <i>SlTCP29</i> double-gene knockout had a lowered transcript level of <i>SlCKX2</i> and an elevated level of cytokinin.</li>\\n \\n \\n <li>This work led to the identification of two key regulators of tomato compound leaf development and their targeted genes involved in cytokinin metabolic pathway. A model of regulation of compound leaf development was proposed based on observations of this study.</li>\\n </ul>\\n \\n </div>\",\"PeriodicalId\":48887,\"journal\":{\"name\":\"New Phytologist\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.4000,\"publicationDate\":\"2023-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Phytologist\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/nph.19221\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/nph.19221","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
SlTCP24 and SlTCP29 synergistically regulate compound leaf development through interacting with SlAS2 and activating transcription of SlCKX2 in tomato
The complexity of compound leaves results primarily from the leaflet initiation and arrangement during leaf development. However, the molecular mechanism underlying compound leaf development remains a central research question.
SlTCP24 and SlTCP29, two plant-specific transcription factors with the conserved TCP motif, are shown here to synergistically regulate compound leaf development in tomato. When both of them were knocked out simultaneously, the number of leaflets significantly increased, and the shape of the leaves became more complex. SlTCP24 and SlTCP29 could form both homodimers and heterodimers, and such dimerization was impeded by the leaf polarity regulator SlAS2, which interacted with SlTCP24 and SlTCP29.
SlTCP24 and SlTCP29 could bind to the TCP-binding cis-element of the SlCKX2 promoter and activate its transcription. Transgenic plants with SlTCP24 and SlTCP29 double-gene knockout had a lowered transcript level of SlCKX2 and an elevated level of cytokinin.
This work led to the identification of two key regulators of tomato compound leaf development and their targeted genes involved in cytokinin metabolic pathway. A model of regulation of compound leaf development was proposed based on observations of this study.
期刊介绍:
New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.