绿唇贻贝Perna Canalitulus中的Perksus olseni:诊断评估、患病率和分布。

IF 1.1 4区 农林科学 Q3 FISHERIES Diseases of aquatic organisms Pub Date : 2023-09-28 DOI:10.3354/dao03750
Henry S Lane, Diana Jaramillo, Mukul Sharma
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引用次数: 0

摘要

Perkinsus olseni(Perkinsidae)是一种软体动物寄生虫,应向世界动物卫生组织报告,据报道,它存在于新西兰的几种贝类宿主中,包括本地的绿唇贻贝Perna canaliculus。绿唇贻贝占新西兰水产养殖出口价值的一半以上,根据广泛的组织学监测,历史上一直被认为没有严重疾病。在绿唇贻贝中发现的P.olseni引发了人们对绿唇贻蚌未来疾病威胁的质疑,特别是在不断变化的海洋气候条件下。使用从养殖(n=358)和野生(n=236)种群中采集的贻贝,我们旨在确定新西兰各地绿唇贻贝中奥氏假单胞菌的分布和流行率,并评估诊断测试的性能,包括实时PCR、常规PCR和使用Ray液体巯基乙酸培养基(RFTM)培养。使用具有信息先验的贝叶斯潜在类别分析来评估患病率和诊断测试性能。除了1个来自港口的野生种群的患病率为22%外,奥氏疟原虫的患病率是0-3%。实时PCR的诊断灵敏度最高(87%),而常规PCR和RFTM分别为62%和21%。所有方法的诊断特异性相似(96-98%)。研究期间未观察到死亡。我们的研究结果表明,实时PCR是最适合在新西兰条件下监测亚临床感染的绿唇贻贝中的奥氏假单胞菌的诊断测试。
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Perkinsus olseni in green-lipped mussels Perna canaliculus: diagnostic evaluation, prevalence, and distribution.

Perkinsus olseni (Perkinsidae) is a molluscan parasite notifiable to the World Organisation for Animal Health that is reported in several shellfish hosts in New Zealand, including the native green-lipped mussel Perna canaliculus. Green-lipped mussels comprise over half of New Zealand's aquaculture export value and have historically been considered free of serious diseases based on extensive histology-based surveillance. The discovery of P. olseni in green-lipped mussels has raised questions about future disease threats to green-lipped mussels, particularly under changing ocean climatic conditions. Using mussels collected from farmed (n = 358) and wild (n = 236) populations, we aimed to determine the distribution and prevalence of P. olseni in green-lipped mussels around New Zealand, and assess the performance of diagnostic tests, including real-time PCR, conventional PCR, and culture using Ray's fluid thioglycolate medium (RFTM). Prevalence and diagnostic test performance was evaluated using Bayesian latent class analysis with informative priors. The prevalence of P. olseni was 0-3%, except for 1 wild population from a harbour where prevalence was 22%. Real-time PCR had the highest diagnostic sensitivity (87%) compared to 62 and 21% for conventional PCR and RFTM, respectively. Diagnostic specificity was similar among all methods (96-98%). No mortality was observed during the study. Our results suggest that real-time PCR is the diagnostic test best suited for surveillance of P. olseni in subclinically infected green-lipped mussels under New Zealand conditions.

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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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