达尔巴万星在临床相关温度下对甲氧西林敏感的金黄色葡萄球菌具有热稳定性。

IF 1.8 Q3 INFECTIOUS DISEASES Journal of Bone and Joint Infection Pub Date : 2023-06-28 eCollection Date: 2023-01-01 DOI:10.5194/jbji-8-175-2023
Aaron K Hoyt, Patrick Lawler, Mathias Bostrom, Alberto V Carli, Ashley E Levack
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摘要

引言:尽管骨科感染率多年来一直保持不变,但随着人口老龄化,医疗系统的负担继续增加。通过聚甲基丙烯酸甲酯骨水泥进行局部抗生素递送是治疗骨和关节感染的常见辅助手段。达尔巴万星是一种与万古霉素同属一类的新型脂糖肽抗生素,在全身使用时对革兰氏阳性菌显示出疗效,但尚未作为局部抗生素进行研究。本研究旨在确定达尔巴万星在聚甲基丙烯酸甲酯水泥聚合过程中的预期温度下是否具有热稳定性。方法:根据先前定义的两种临床相关模型中的固化温度模型,制备达尔巴万星储备溶液,并使用聚合酶链式反应机加热: mm聚甲基丙烯酸甲酯珠和聚甲基丙烷关节膝部垫片模型。然后转移加热的dalbavancin的等分试样以在核心体温下孵育(37 ∘C) 并在截至28的不同时间点进行分析 d.90 % 使用标准microbroth稀释测定法针对甲氧西林敏感的金黄色葡萄球菌(American Type Culture Collection,ATCC,0173K)测定每个加热样品的菌落被抑制(MIC90)。结果:达巴万星的MIC90平均值为1.63 µgmL-1±0.49对抗0173K金黄色葡萄球菌。与未加热的对照组相比,两种模型中加热达尔巴万星后的相对MIC90值均无显著差异。结论:达尔巴万星在聚甲基丙烯酸甲酯水泥的固化温度和人体核心体温超过28℃时是热稳定的 d.未来的体外和体内研究有必要在临床使用前进一步研究达尔巴万星作为局部抗生素的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Dalbavancin is thermally stable at clinically relevant temperatures against methicillin-sensitive Staphylococcus Aureus.

Introduction: While the rate of orthopaedic infections has remained constant over the years, the burden on healthcare systems continues to rise with an aging population. Local antibiotic delivery via polymethyl methacrylate bone cement is a common adjunct in treating bone and joint infections. Dalbavancin is a novel lipoglycopeptide antibiotic in the same class as vancomycin that has shown efficacy against Gram-positive organisms when used systemically but has not been investigated as a local antibiotic. This study aims to identify whether dalbavancin is thermally stable at the temperatures expected during the polymerization of polymethyl methacrylate cement. Methods: Stock solutions of dalbavancin were prepared and heated using a polymerase chain reaction machine based upon previously defined models of curing temperatures in two clinically relevant models: a 10 mm polymethyl methacrylate bead and a polymethyl methacrylate articulating knee spacer model. Aliquots of heated dalbavancin were then transferred to be incubated at core body temperature (37 C) and analyzed at various time points up to 28 d. The minimum inhibitory concentration at which 90 % of colonies were inhibited (MIC90) for each heated sample was determined against methicillin-sensitive Staphylococcus aureus (American Type Culture Collection, ATCC, 0173K) using a standard microbroth dilution assay. Results: The average MIC90 of dalbavancin was 1.63 µgmL-1 ±0.49 against 0173K S. aureus. There were no significant differences in the relative MIC90 values after heating dalbavancin in either model compared to unheated control dalbavancin. Conclusions: Dalbavancin is thermally stable at the curing temperatures of polymethyl methacrylate cement and at human core body temperature over 28 d. Future in vitro and in vivo studies are warranted to further investigate the role of dalbavancin as a local antibiotic prior to its clinical use.

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CiteScore
3.70
自引率
0.00%
发文量
29
审稿时长
12 weeks
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