开发一种新的基于凝集素的金纳米粒子护理点免疫测定法,用于快速诊断严重登革热感染患者。

Q2 Health Professions Journal of immunoassay & immunochemistry Pub Date : 2023-11-02 Epub Date: 2023-10-31 DOI:10.1080/15321819.2023.2260480
Moumita Paul, Bibhuti Saha, Sumi Mukhopadhyay
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引用次数: 0

摘要

对严重登革热感染患者的快速诊断有助于对登革热病毒引起的病例进行有效的临床管理。侧流免疫测定法(LFIA)由于其人眼读数快、使用简单且价格低廉,已被广泛用于登革热的快速诊断。尽管有几种商业登革热护理点检测方法,但没有一种能成功区分严重和非严重形式的登革热感染。在目前的研究中,首次报道了一种新的基于凝集素的护理点检测方法,该方法以镀金片作为检测标签,用于早期检测严重登革热感染患者。在该试验中,通过检测已知登革热严重程度标志物玻璃体凝集素的糖基化特征来诊断登革热严重程度。使用了两种凝集素,即DSA(曼陀罗)和MAA(马齿苋),它们可以识别(α2-3)连锁中的半乳糖Gal-(1-4)GlcNAc和唾液酸等特定聚糖,表现出高灵敏度和高特异性,DSA分别为90%和85%,MAA分别为90.91%和95%。这种新的检测方法的检测限为5 µgµl-1,并能快速(30 min)和对严重登革热病例的敏感检测。报道的护理点免疫测定法在早期识别登革热严重程度患者方面显示出相当大的前景。
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Development of a novel lectin-based gold nanoparticle point-of-care immunoassay for rapid diagnosis of patients with severe Dengue infection.

Rapid diagnosis of patients with severe Dengue infection can be useful for the efficient clinical management of cases caused by the Dengue virus. Lateral Flow Immunoassay (LFIA) have been broadly used for rapid Dengue diagnosis, because of their quick readouts with the human eye, simplicity of use, and affordability. Despite the availability of several commercial Dengue point-of-care assays, none has shown to be successful in discriminating between severe and nonsevere forms of Dengue infection. In the current study, for the first time, a novel lectin-based point-of-care assay for the early detection of patients with severe Dengue infection with gold-adorned sheets as detection labels is being reported. In this assay, Dengue severity was diagnosed by detecting the glycosylation profile of vitronectin, a known Dengue severity marker. Two lectins were employed namely DSA (Datura stramonium) and MAA (Maackia amurensis) that can recognize specific glycans like galactose Gal-(1-4) GlcNAc and sialic acid in an (α2-3) linkage, which displayed high sensitivity and high specificity, i.e. 90% and 85% for DSA and 90.91% and 95% for MAA. The new assay has a detection limit of 5 µg µl-1 and enables the quick (30 min) and sensitive detection of severe Dengue cases. The reported point-of-care immunoassay exhibits considerable promise for early identification of patients with Dengue severity.

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来源期刊
CiteScore
3.50
自引率
0.00%
发文量
38
审稿时长
>12 weeks
期刊介绍: The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.
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