类葡聚糖合成酶2是水稻种子发育、灌浆和花粉育性所必需的。

IF 4.8 1区 农林科学 Q1 AGRONOMY Rice Pub Date : 2023-10-07 DOI:10.1186/s12284-023-00662-z
Ronghua Qiu, Yang Liu, Zhengzheng Cai, Jieqiong Li, Chunyan Wu, Gang Wang, Chenchen Lin, Yulin Peng, Zhanlin Deng, Weiqi Tang, Weiren Wu, Yuanlin Duan
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摘要

背景:葡聚糖合成酶样(GSL)基因在植物中涉及胼胝质合成和沉积的一些重要的高度专业化的发育和细胞过程中是必不可少的。目前,GSL基因最具特征的生殖功能是花粉形成和卵巢扩张,但它们在种子启动中的作用尚不清楚。结果:我们鉴定了一个水稻种子突变体,水性种子1-1(ws1-1),该突变体含有OsGSL2基因突变。突变体产生的种子缺乏胚胎和胚乳,但充满透明且富含蔗糖的液体。在ws1-1小穗中,胚珠发育正常,但小孢子发生和雄配子体发育受到影响,导致可育花粉减少。受精后,虽然种皮正常发育,但胚胎未能正常分化。此外,分裂的胚乳游离核没有迁移到胚囊的外围,而是聚集在一起,从而阻止了它们的增殖和细胞化。此外,在ws1-1中,珠心细胞的变性被延迟。OsGSL2在生殖器官和发育中的种子中高度表达。破坏OsGSL2减少了小孢子外壁上的胼胝质沉积,并在发育中的颖果中破坏了环状胼胝质鞘的形成,导致花粉缺陷和种子败育。结论:我们的研究结果表明,OsGSL2对水稻育性至关重要,是胚胎分化和胚乳游离核定位所必需的,这表明胼胝质合成酶基因OsGSL2在种子启动中发挥着独特的作用,这为谷物种子发育的调控提供了新的见解。
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Glucan Synthase-like 2 is Required for Seed Initiation and Filling as Well as Pollen Fertility in Rice.

Background: The Glucan synthase-like (GSL) genes are indispensable for some important highly-specialized developmental and cellular processes involving callose synthesis and deposition in plants. At present, the best-characterized reproductive functions of GSL genes are those for pollen formation and ovary expansion, but their role in seed initiation remains unknown.

Results: We identified a rice seed mutant, watery seed 1-1 (ws1-1), which contained a mutation in the OsGSL2 gene. The mutant produced seeds lacking embryo and endosperm but filled with transparent and sucrose-rich liquid. In a ws1-1 spikelet, the ovule development was normal, but the microsporogenesis and male gametophyte development were compromised, resulting in the reduction of fertile pollen. After fertilization, while the seed coat normally developed, the embryo failed to differentiate normally. In addition, the divided endosperm-free nuclei did not migrate to the periphery of the embryo sac but aggregated so that their proliferation and cellularization were arrested. Moreover, the degeneration of nucellus cells was delayed in ws1-1. OsGSL2 is highly expressed in reproductive organs and developing seeds. Disrupting OsGSL2 reduced callose deposition on the outer walls of the microspores and impaired the formation of the annular callose sheath in developing caryopsis, leading to pollen defect and seed abortion.

Conclusions: Our findings revealed that OsGSL2 is essential for rice fertility and is required for embryo differentiation and endosperm-free nucleus positioning, indicating a distinct role of OsGSL2, a callose synthase gene, in seed initiation, which provides new insight into the regulation of seed development in cereals.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
期刊最新文献
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