通过开发用于眼科手术的球周麻醉技术来改善大鼠的福利:一项初步研究。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-09-20 DOI:10.1177/00236772231178433
Ady Eliav, Ron Ofri, Kelsey Brust, Yishai Kushnir, Yael Shilo-Benjamini
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引用次数: 0

摘要

大鼠是研究青光眼发病机制和新治疗方法的常用动物模型,该模型是通过侵入性疼痛程序进行实验诱导的。在眼科手术之前,人们和家畜经常使用球周麻醉(PBA)来提供良好的围手术期镇痛。我们的目标是开发一种适用于大鼠解剖结构的PBA技术,改善用作青光眼模型的动物的福利。18具大鼠尸体(n = 36眼)来确定最佳的针头插入位置。使用0.1的五种注射技术 毫升/100 g利多卡因2%和造影剂(体积比1:1)通过计算机断层扫描(CT)进行比较。CT图像对四个位置的注射分布进行了评分:视神经外、视神经内、视神经周围和眶裂(0-8,其中0 = 无和8 = 优秀)。使用背内侧-75°(5;范围2-6)和内眼角(4.5;范围2-8)注射技术的中位得分与背内侧-45°(4;范围3-6)技术没有不同,并且与中腹(3;范围2-5)和腹外侧(2;范围1-3)技术相比更高(分布更好)。两种优越的技术用于两个实验大鼠(n = 4眼),以确定影响角膜接触阈值(CTT)和眼周皮肤敏感性(PSS)所需的0.5%布比卡因的体积。0.05的体积 毫升/100 g使CTT和PSS降低数小时,而较大的体积会产生过长的影响。背内侧-75°或内眼角PBA使用0.05 毫升/100 g布比卡因可能为大鼠提供眼部和眼周镇痛,有轻微的短暂不良反应。
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Improving rat welfare through the development of a peribulbar anaesthesia technique for ophthalmic procedures: A preliminary study.

Rats are a commonly used animal model for the study of the pathogenesis and novel treatments of glaucoma, which is induced experimentally using invasive, painful procedures. Peribulbar anaesthesia (PBA) is frequently used in people and domestic animals prior to ophthalmic surgeries to provide excellent perioperative analgesia. Our goal was to develop a PBA technique adapted to rat anatomy, improving the welfare of animals used as a model for glaucoma. Eighteen rat cadavers (n = 36 eyes) were used to establish the optimal needle insertion location. Five injection techniques using 0.1 mL/100 g lidocaine 2% and a contrast agent (1:1 volume ratio) were compared via computed tomography (CT). CT images were scored for injectate distribution at four locations: extraconal, intraconal, around the optic nerve and at the orbital fissure (scale 0-8, where 0 = none and 8 = excellent). Median scores using the dorso-medial-75° (5; range 2-6) and medial-canthus (4.5; range 2-8) injection techniques were not different from the dorso-medial-45° (4; range 3-6) technique and were higher (better distribution) compared with mid-ventral (3; range 2-5) and ventro-lateral (2; range 1-3) techniques. The two superior techniques were used in two experimental rats (n = 4 eyes) to determine the volume of bupivacaine 0.5% necessary to affect corneal touch threshold (CTT) and periocular skin sensitivity (PSS). A volume of 0.05 mL/100 g decreased CTT and PSS for several hours, while a larger volume produced excessively long effects. Dorso-medial-75° or medial-canthus PBA using 0.05 mL/100 g bupivacaine are likely to provide ocular and periocular analgesia in rats, with minor transient adverse effects.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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