{"title":"疾病建模和药物发现中的视网膜类器官:机遇与挑战。","authors":"Koushik Chakrabarty PhD , Divyani Nayak MSc , Jayasree Debnath MTech , Debashish Das PhD , Rohit Shetty DNB, FRCS, PhD , Arkasubhra Ghosh PhD","doi":"10.1016/j.survophthal.2023.09.003","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Diseases leading to retinal cell loss can cause severe visual impairment<span> and blindness<span><span>. The lack of effective therapies<span> to address retinal cell loss and the absence of intrinsic regeneration in the human retina leads to an irreversible pathological condition. Progress in recent years in the generation of human three-dimensional retinal organoids from </span></span>pluripotent stem cells<span><span> makes it possible to recreate the cytoarchitecture and associated cell-cell interactions of the human retina in remarkable detail. These human three-dimensional retinal organoid systems made of distinct retinal cell types and possessing contextual </span>physiological responses allow the study of human </span></span></span></span>retina development and </span>retinal disease<span> pathology in a way animal model and two-dimensional cell cultures were unable to achieve. We describe the derivation of retinal organoids from human pluripotent stem cells and their application for modeling retinal disease pathologies, while outlining the opportunities and challenges for its application in academia and industry.</span></p></div>","PeriodicalId":22102,"journal":{"name":"Survey of ophthalmology","volume":"69 2","pages":"Pages 179-189"},"PeriodicalIF":5.1000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Retinal organoids in disease modeling and drug discovery: Opportunities and challenges\",\"authors\":\"Koushik Chakrabarty PhD , Divyani Nayak MSc , Jayasree Debnath MTech , Debashish Das PhD , Rohit Shetty DNB, FRCS, PhD , Arkasubhra Ghosh PhD\",\"doi\":\"10.1016/j.survophthal.2023.09.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>Diseases leading to retinal cell loss can cause severe visual impairment<span> and blindness<span><span>. The lack of effective therapies<span> to address retinal cell loss and the absence of intrinsic regeneration in the human retina leads to an irreversible pathological condition. Progress in recent years in the generation of human three-dimensional retinal organoids from </span></span>pluripotent stem cells<span><span> makes it possible to recreate the cytoarchitecture and associated cell-cell interactions of the human retina in remarkable detail. These human three-dimensional retinal organoid systems made of distinct retinal cell types and possessing contextual </span>physiological responses allow the study of human </span></span></span></span>retina development and </span>retinal disease<span> pathology in a way animal model and two-dimensional cell cultures were unable to achieve. We describe the derivation of retinal organoids from human pluripotent stem cells and their application for modeling retinal disease pathologies, while outlining the opportunities and challenges for its application in academia and industry.</span></p></div>\",\"PeriodicalId\":22102,\"journal\":{\"name\":\"Survey of ophthalmology\",\"volume\":\"69 2\",\"pages\":\"Pages 179-189\"},\"PeriodicalIF\":5.1000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Survey of ophthalmology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039625723001273\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OPHTHALMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Survey of ophthalmology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039625723001273","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
Retinal organoids in disease modeling and drug discovery: Opportunities and challenges
Diseases leading to retinal cell loss can cause severe visual impairment and blindness. The lack of effective therapies to address retinal cell loss and the absence of intrinsic regeneration in the human retina leads to an irreversible pathological condition. Progress in recent years in the generation of human three-dimensional retinal organoids from pluripotent stem cells makes it possible to recreate the cytoarchitecture and associated cell-cell interactions of the human retina in remarkable detail. These human three-dimensional retinal organoid systems made of distinct retinal cell types and possessing contextual physiological responses allow the study of human retina development and retinal disease pathology in a way animal model and two-dimensional cell cultures were unable to achieve. We describe the derivation of retinal organoids from human pluripotent stem cells and their application for modeling retinal disease pathologies, while outlining the opportunities and challenges for its application in academia and industry.
期刊介绍:
Survey of Ophthalmology is a clinically oriented review journal designed to keep ophthalmologists up to date. Comprehensive major review articles, written by experts and stringently refereed, integrate the literature on subjects selected for their clinical importance. Survey also includes feature articles, section reviews, book reviews, and abstracts.