疾病建模和药物发现中的视网膜类器官:机遇与挑战。

IF 5.1 2区 医学 Q1 OPHTHALMOLOGY Survey of ophthalmology Pub Date : 2024-03-01 DOI:10.1016/j.survophthal.2023.09.003
Koushik Chakrabarty PhD , Divyani Nayak MSc , Jayasree Debnath MTech , Debashish Das PhD , Rohit Shetty DNB, FRCS, PhD , Arkasubhra Ghosh PhD
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引用次数: 0

摘要

导致视网膜细胞损失的疾病可导致严重的视觉损伤和失明。缺乏有效的治疗方法来解决视网膜细胞损失和人类视网膜缺乏内在再生的问题,导致了不可逆转的病理状况。近年来,在从多能干细胞生成人类三维(3D)视网膜类器官(RO)方面取得了进展,这使得能够非常详细地重建人类视网膜的细胞结构和相关的细胞-细胞相互作用。这些由不同视网膜细胞类型制成并具有上下文生理反应的人类3D RO系统允许以动物模型和二维细胞培养无法实现的方式研究人类视网膜发育和视网膜疾病(RD)病理。在这篇综述中,我们描述了从人类多能干细胞中提取RO及其在RD病理建模中的应用,同时概述了其在学术界和工业界应用的机遇和挑战。
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Retinal organoids in disease modeling and drug discovery: Opportunities and challenges

Diseases leading to retinal cell loss can cause severe visual impairment and blindness. The lack of effective therapies to address retinal cell loss and the absence of intrinsic regeneration in the human retina leads to an irreversible pathological condition. Progress in recent years in the generation of human three-dimensional retinal organoids from pluripotent stem cells makes it possible to recreate the cytoarchitecture and associated cell-cell interactions of the human retina in remarkable detail. These human three-dimensional retinal organoid systems made of distinct retinal cell types and possessing contextual physiological responses allow the study of human retina development and retinal disease pathology in a way animal model and two-dimensional cell cultures were unable to achieve. We describe the derivation of retinal organoids from human pluripotent stem cells and their application for modeling retinal disease pathologies, while outlining the opportunities and challenges for its application in academia and industry.

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来源期刊
Survey of ophthalmology
Survey of ophthalmology 医学-眼科学
CiteScore
10.30
自引率
2.00%
发文量
138
审稿时长
14.8 weeks
期刊介绍: Survey of Ophthalmology is a clinically oriented review journal designed to keep ophthalmologists up to date. Comprehensive major review articles, written by experts and stringently refereed, integrate the literature on subjects selected for their clinical importance. Survey also includes feature articles, section reviews, book reviews, and abstracts.
期刊最新文献
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