甲硫氨酸脑啡肽(MENK)在抗流感反应中上调记忆T细胞。

IF 2.9 4区 医学 Q3 IMMUNOLOGY BMC Immunology Pub Date : 2023-10-12 DOI:10.1186/s12865-023-00573-0
Jing Tian, Wenrui Fu, Zifeng Xie, Xiaonan Wang, Miao Miao, Fengping Shan, Xiaodong Yu
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摘要

预防流感病毒的新型预防药物和疫苗接种策略应在肺气道和外周淋巴器官中诱导特异性效应T细胞免疫反应。设计促进T细胞介导的反应和记忆T细胞分化的方法将增强宿主对呼吸道传染病的抵抗力。本研究的结果表明,通过鼻内给药的MENK肺部给药降低了病毒滴度,上调了阿片受体MOR和DOR,增加了支气管肺泡灌洗液和肺部T细胞亚群的比例,包括CD8+T细胞、CD8+TEM细胞、NP/PA效应CD8+TEM细胞,以及淋巴结中的CD4+/CD8+TCM细胞,以保护小鼠免受流感病毒攻击。此外,我们证明,在感染的第10天,CD4+TM和CD8+TM细胞的比例显著增加,这意味着在流感感染的早期阶段建立了稳定的TCM和TEM谱系。总之,我们的数据表明,类似于甲型流感病毒的自然感染途径,鼻内给药MENK可以通过上调T细胞介导的针对流感病毒的适应性免疫反应来发挥抗病毒活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Methionine enkephalin(MENK) upregulated memory T cells in anti-influenza response.

Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8+ T cells, CD8+ TEM cells, NP/PA-effector CD8+ TEM cells in bronchoalveolar lavage fluid and lungs, and CD4+/CD8+ TCM cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4+ TM and CD8+ TM cells were significantly increased, which meant that a stable TCM and TEM lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.

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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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