{"title":"甲硫氨酸脑啡肽(MENK)在抗流感反应中上调记忆T细胞。","authors":"Jing Tian, Wenrui Fu, Zifeng Xie, Xiaonan Wang, Miao Miao, Fengping Shan, Xiaodong Yu","doi":"10.1186/s12865-023-00573-0","DOIUrl":null,"url":null,"abstract":"<p><p>Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8<sup>+</sup> T cells, CD8<sup>+</sup> T<sub>EM</sub> cells, NP/PA-effector CD8<sup>+</sup> T<sub>EM</sub> cells in bronchoalveolar lavage fluid and lungs, and CD4<sup>+</sup>/CD8<sup>+</sup> T<sub>CM</sub> cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4<sup>+</sup> T<sub>M</sub> and CD8<sup>+</sup> T<sub>M</sub> cells were significantly increased, which meant that a stable T<sub>CM</sub> and T<sub>EM</sub> lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":"24 1","pages":"38"},"PeriodicalIF":2.9000,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571428/pdf/","citationCount":"0","resultStr":"{\"title\":\"Methionine enkephalin(MENK) upregulated memory T cells in anti-influenza response.\",\"authors\":\"Jing Tian, Wenrui Fu, Zifeng Xie, Xiaonan Wang, Miao Miao, Fengping Shan, Xiaodong Yu\",\"doi\":\"10.1186/s12865-023-00573-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8<sup>+</sup> T cells, CD8<sup>+</sup> T<sub>EM</sub> cells, NP/PA-effector CD8<sup>+</sup> T<sub>EM</sub> cells in bronchoalveolar lavage fluid and lungs, and CD4<sup>+</sup>/CD8<sup>+</sup> T<sub>CM</sub> cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4<sup>+</sup> T<sub>M</sub> and CD8<sup>+</sup> T<sub>M</sub> cells were significantly increased, which meant that a stable T<sub>CM</sub> and T<sub>EM</sub> lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.</p>\",\"PeriodicalId\":9040,\"journal\":{\"name\":\"BMC Immunology\",\"volume\":\"24 1\",\"pages\":\"38\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2023-10-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571428/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12865-023-00573-0\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12865-023-00573-0","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Methionine enkephalin(MENK) upregulated memory T cells in anti-influenza response.
Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8+ T cells, CD8+ TEM cells, NP/PA-effector CD8+ TEM cells in bronchoalveolar lavage fluid and lungs, and CD4+/CD8+ TCM cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4+ TM and CD8+ TM cells were significantly increased, which meant that a stable TCM and TEM lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.
期刊介绍:
BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.