高强度间歇训练可改善2型糖尿病雄性大鼠下丘脑食欲调节:瘦素的作用。

IF 3.6 4区 医学 Q3 CELL BIOLOGY Cellular and Molecular Neurobiology Pub Date : 2023-11-01 Epub Date: 2023-10-12 DOI:10.1007/s10571-023-01421-w
Kayvan Khoramipour, Maryam Hossein Rezaei, Elham Madadizadeh, Mahdieh Sadat Hosseini, Zahra Soltani, Janis Schierbauer, Othmar Moser
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引用次数: 0

摘要

2型糖尿病(T2D)引起的下丘脑瘦素(LEP)信号的破坏会损害食欲调节。本研究的目的是研究高强度间歇训练(HIIT)对T2D大鼠食欲调节的改善是否可以通过LEP信号介导。在本研究中,20只雄性Wister大鼠被随机分配到四组中的一组:CO(非2型糖尿病对照组)、T2D(2型糖尿病)、EX(非2类型糖尿病运动组)和T2D + EX(2型糖尿病 + 为了诱导T2D,给予2个月的高脂肪饮食和单剂量链脲佐菌素(35mg/kg)的组合。EX和T2D大鼠 + EX组以其最大速度(Vmax)的80-100%进行4-10次跑步机跑步。稳态模型评估胰岛素抵抗(HOMA-IR)、血清胰岛素(INS)和LEP(LEPS)水平以及下丘脑LEP受体(LEP-R)、Janus激酶2(JAK-2)、信号转导子和转录激活子3(STAT-3)、神经肽Y(NPY)、促食欲相关蛋白(AGRP)、,细胞因子信号传导抑制剂(SOCS3)、叉头盒蛋白O1(FOXO1)。ANOVA和Tukey事后检验用于比较两组之间的结果。研究发现,在T2D中,下丘脑中的LEPS和INS水平以及LEP-R、JAK-2、STAT-3、POMC和CART水平较高 + EX组与T2D组相比。另一方面,T2D患者的HOMA-IR、NPY、AGRP、SOCS3和FOXO1水平较低 + EX组与T2D组比较(P
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High Intensity Interval Training can Ameliorate Hypothalamic Appetite Regulation in Male Rats with Type 2 Diabetes: The Role of Leptin.

Disruption of leptin (LEP) signaling in the hypothalamus caused by type 2 diabetes (T2D) can impair appetite regulation. The aim of this study was to investigate whether the improvement in appetite regulation induced by high-intensity interval training (HIIT) in rats with T2D can be mediated by LEP signaling. In this study, 20 male Wister rats were randomly assigned to one of four groups: CO (non-type 2 diabetes control), T2D (type 2 diabetes), EX (non-type 2 diabetes exercise), and T2D + EX (type 2 diabetes + exercise).To induce T2D, a combination of a high-fat diet for 2 months and a single dose of streptozotocin (35 mg/kg) was administered. Rats in the EX and T2D + EX groups performed 4-10 intervals of treadmill running at 80-100% of their maximum velocity (Vmax). Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), serum levels of insulin (INS) and LEP (LEPS) as well as hypothalamic expression of LEP receptors (LEP-R), Janus kinase 2 (JAK-2), signal transducer and activator of transcription 3 (STAT-3), neuropeptide Y (NPY), agouti-related protein (AGRP), pro-opiomelanocortin cocaine (POMC), amphetamine-related transcript (CART), suppressor of cytokine signaling (SOCS3), forkhead box protein O1 (FOXO1) were assessed. ANOVA and Tukey post hoc tests were used to compare the results between the groups. The levels of LEPS and INS, as well as the levels of LEP-R, JAK-2, STAT-3, POMC, and CART in the hypothalamus were found to be higher in the T2D + EX group compared to the T2D group. On the other hand, the levels of HOMA-IR, NPY, AGRP, SOCS3, and FOXO1 were lower in the T2D + EX group compared to the T2D group (P < 0.0001). The findings of this study suggest that HIIT may improve appetite regulation in rats with T2D, and LEP signaling may play a crucial role in this improvement. Graphical abstract (leptin signaling in the hypothalamus), Leptin (LEP), Leptin receptor (LEP-R), Janus kinase 2 (JAK2), Signal transducer and activator of transcription 3 (STAT3), expressing Neuropeptide Y (NPY), Agouti-related protein (AGRP), anorexigenic neurons (expressing pro-opiomelanocortin cocaine (POMC), Amphetamine-related transcript (CART), suppressor of cytokine signaling (SOCS3), forkhead box protein O1 (FOXO1).

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来源期刊
CiteScore
7.70
自引率
0.00%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Cellular and Molecular Neurobiology publishes original research concerned with the analysis of neuronal and brain function at the cellular and subcellular levels. The journal offers timely, peer-reviewed articles that describe anatomic, genetic, physiologic, pharmacologic, and biochemical approaches to the study of neuronal function and the analysis of elementary mechanisms. Studies are presented on isolated mammalian tissues and intact animals, with investigations aimed at the molecular mechanisms or neuronal responses at the level of single cells. Cellular and Molecular Neurobiology also presents studies of the effects of neurons on other organ systems, such as analysis of the electrical or biochemical response to neurotransmitters or neurohormones on smooth muscle or gland cells.
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