分析海水中微藻盐酸成分

Liska Ramdanawati, D. Kurnia, Vita Aji Kusumaning Tyas, Z. Nurachman
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引用次数: 4

摘要

印度尼西亚是一个具有潜在生物多样性的水生国家。微藻是潜在的生物多样性之一。微藻富含脂肪酸,具有开发功能性食品的潜力。硅藻中发现的最多的脂肪酸是肉豆蔻酸、棕榈酸、DHA和EPA。盐渍硅藻是一种富含脂肪酸的海洋微藻。本研究旨在分析海洋微藻Navicula salinicola的脂肪酸组成。盐碱Navicula salinicola在含2.5的Guillard培养基中培养;5;氮含量为7.5%,培养11天。从收获中获得的干生物质用于提取。微藻脂质提取采用Bligh & Dyer法,提取率为9.9%;14.5%和17.5%的产油率。GC-FID分析结果鉴定出6种脂肪酸分别为棕榈酸、肉豆蔻酸、五酸、硬脂酸、棕榈油酸和二十碳五烯酸、EPA。脂肪酸含量最高的是棕榈酸,为64.04%;65.03%;和53.55%。
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ANALISIS KOMPOSISI ASAM LEMAK DARI MIKROALGA LAUT Navicula salinicola
Indonesia is an aquatic country with potential biodiversity. One of the potential biodiversity is microalgae. Microalgae potential to be developed as a functional food with the characteristics of its fatty acids. The most fatty acids found in diatoms are myristic acid, palmitic acid, DHA and EPA. One of marine microalgae contain lot of fatty acids is diatom Navicula salinicola. This study was aim to analyze fatty acid composition of marine microalgae Navicula salinicola. Navicula salinicola was cultivated in Guillard medium with 2.5; 5; and 7.5 % of nitrogen composition and cultivated for 11 days. Dry biomass obtained from harvesting was used for the extraction. Extraction of lipid from microalgae used Bligh & Dyer method with 9,9%; 14.5%, and 17.5% of oil yield. Result of GC-FID analysis that were 6 types of fatty acids that identified are palmitic acid, myristic acid, pentadecanoic acid, stearic acid, palmitoleic acid and eicosapentanoic acid, EPA. The highest fatty acid was palmitic acid with fatty acids percentage were 64.04%; 65.03%; and 53.55% respectively.
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审稿时长
24 weeks
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