移植的下鼻甲来源的干细胞在小鼠退化视网膜中向杆状光感受器分化的光镜证据

Q3 Immunology and Microbiology Applied Microscopy Pub Date : 2020-06-03 DOI:10.1186/s42649-020-00031-w

Yong Soo Park, Yeonji Kim, Sung Won Kim, In-Beom Kim

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引用次数: 0

摘要

用差干涉对比(DIC)滤光片共聚焦显微镜观察了经dii标记的人鼻甲源性间充质干细胞(hTMSCs)移植到变性小鼠视网膜下间隙的视网膜垂直切片，并对视紫红质(杆状光感受器的标志物)进行了处理。图像清楚地显示，dii标记的hTMSC具有视紫红质免疫反应附属物，表明移植的hTMSC向杆状光感受器分化。最后，这一发现提示hTMSCs在视网膜变性中的治疗潜力。

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Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse

The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

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来源期刊

Applied Microscopy Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： Applied Microscopy is a peer-reviewed journal sponsored by the Korean Society of Microscopy. The journal covers all the interdisciplinary fields of technological developments in new microscopy methods and instrumentation and their applications to biological or materials science for determining structure and chemistry. ISSN: 22875123, 22874445.