骨髓间充质干细胞(BMSC)外泌体来源的mR-512-5p抑制胶质母细胞瘤细胞的增殖和凋亡

Feng Qiu, Leyi Xu, Li Gong, Lingjun Kong, Jue Zhang, Z. Fei
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引用次数: 0

摘要

本研究探讨来自BMSC的miR-512-5p抑制GBM增殖和促进细胞凋亡的机制。超离心获得BMSC外泌体,透射电镜观察。Western Blot检测CD63和HSP70阳性表达。将GBM细胞系LN229分为WM组、NC组和ZR组,MTT法分析细胞增殖、Tranwell室法分析细胞侵袭能力、流式细胞仪分析细胞凋亡率、Western Blot分析JAG1和notch2的表达。LN229细胞中miR-512-5p水平明显低于U87MG和SHG44细胞。外泌体中CD63、HSP70表达阳性。药物对LN229细胞增殖有抑制作用。与WM组和NC组相比,ZR组细胞增殖率和侵袭量较低,凋亡率较高。ZR组JAG1和notch2蛋白表达量较WM组和NC组降低(P < 0.05)。由此可见,BMSC衍生的miR-512-5p通过靶向JAG1可抑制GBM细胞增殖,增加细胞凋亡。为GBM的治疗提供了一种全新的治疗策略。
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Bone Marrow Mesenchymal Stem Cells (BMSC) Exosome-Derived mR-512-5p Inhibits the Proliferation and Apoptosis of Glioblastoma Cells
This study discusses the mechanism of miR-512-5p derived from BMSC in restraining the proliferation and prompting apoptosis of GBM. BMSC exosome was obtained through ultra-centrifugation and assessed by TEM. The positive presentation of CD63 and HSP70 was detected with Western Blot. The GBM cell line LN229 was divided into WM set, NC set, and ZR set followed by analysis of cell proliferation by MTT method, invasive ability by Tranwell chamber, apoptotic rate by FCM and the expression of JAG1 and notch2 by Western Blot. miR-512-5p level in LN229 cells was significantly lower than U87MG and SHG44 cells. There was positive expression of CD63 and HSP70 in exosome. LN229 cell proliferation was restrained by the drug. ZR set had lower cell proliferation rate and invasive quantity and higher apoptotic rate than WM set and NC set. The protein expressions of JAG1 and notch2 in ZR set was reduced compared with WM set and NC set (P <0.05) without difference between NC set and WM set (P >0.05). In conclusion, GBM cell proliferation could be restrained and apoptosis could be increased by miR-512-5p derived from BMSC through targeting JAG1. It could provide a brand-new therapeutic strategy for the treatment on GBM.
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