粘球孢离体培养及对欧洲槲寄生的生物防治潜力

IF 1.1 4区 生物学 Q3 PLANT SCIENCES Acta Botanica Croatica Pub Date : 2023-05-31 DOI:10.37427/botcro-2023-011
Yu. O. Bilonozhko, T. Krupodorova, A. Rabokon, A. Postovoitova, L. Kalafat, Y. Pirko, Y. Blume
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引用次数: 0

摘要

为了改进白槲寄生(欧洲槲寄生)的生物防治,需要更多关于内脏球孢菌感染、其基本营养需求、生长和体外生长特性的详细信息。本研究的目的是从桃花楸中分离和鉴定与Viscumаlbum相关的真菌B.visci,提供其体外引入和在不同培养基上生长特性的信息,并测试分离真菌的致病性。为了实现这些目标,通过显微镜对槲寄生感染部位的分生孢子的形态进行了评估。从新采集的V.album中分离得到的菌株被鉴定为内脏球孢菌的变形球孢菌。营养菌丝体的形态和B.visci的生长因所用培养基的不同而不同。燕麦是支持生长和产孢的最佳培养基。欧洲槲寄生在实验室条件下的再感染在液体培养基和田间条件下显示出阳性结果,但仅在槲寄生叶片受到机械损伤后。我们的研究结果扩展了关于这种真菌的最佳培养的知识。这可能有助于进一步对内脏B.visci分离株进行真菌学和病理学研究,其结果为实施槲寄生防治措施提供了理论依据。
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In vitro cultivation and biocontrol potential of Botryosphaeria visci against European mistletoe (Viscum album L.)
To improve the biological control of white mistletoe (European mistletoe) additional detailed information on Botryosphaeria visci infection, its basic nutritional requirements, growth, and in vitro growth characteristics is needed. The objectives of this study were to isolate and identify the fungus B. visci associated with Viscum аlbum from Sorbus aucuparia, to provide information on its in vitro introduction and growth characteristics on different media, and to test the pathogenicity of the isolated fungus. To achieve these goals, the morphology of conidiophores from infected parts of mistletoe was evaluated by microscopy. The isolate from fresh collections of V. album was identified as Sphaeropsis visci anamorph of ascomycete Botryosphaeria visci. The morphology of the vegetative mycelium and growth of B. visci varied depending on the media used. The best medium supporting growth and sporulation was oatmeal. Re-infection of European mistletoe in laboratory conditions showed positive result on liquid media, and in field conditions but only after mechanical damage to the mistletoe leaves. Our results expand the knowledge regarding the optimal cultivation of this fungus. This may facilitate further mycological and pathological studies involving B. visci isolates, and the results have a theoretical basis for the implementation of measures for the prevention and control of mistletoe.
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来源期刊
Acta Botanica Croatica
Acta Botanica Croatica PLANT SCIENCES-
CiteScore
2.50
自引率
0.00%
发文量
34
审稿时长
>12 weeks
期刊介绍: The interest of the journal is field (terrestrial and aquatic) and experimental botany (including microorganisms, plant viruses, bacteria, unicellular algae), from subcellular level to ecosystems. The attention of the Journal is aimed to the research of karstic areas of the southern Europe, karstic waters and the Adriatic Sea (Mediterranean).
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