研究了不同提取方法对木木屑精油产率、化学成分及抗真菌活性的影响

Chaimaa Bouyahia, M. Benbouzi̇d, S. El hajjaji, M. Slaoui̇, F. Bentata, M. Labhi̇li̇li̇, A. Bouyahya
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引用次数: 0

摘要

本工作的目的是确定提取方法对摩洛哥大西洋雪松锯末中雪松精油的产量、化学成分和抗真菌活性的影响。EOs通过不同的方法提取:水溶、索氏、浸渍和超声。使用气相色谱-质谱(GC-MS)分析来测定化学成分。加氢蒸馏、索氏蒸馏、浸渍和超声的EOs产率分别为5.60%、11.68%、4.82%和9.33%。GC-MS揭示了不同提取方法的化合物的多样性。事实上,通过索氏法、浸渍法和超声法获得的EOs的主要化合物是共聚酸甲酯,其比率分别为28.41%、20.24%和24.17%。然而-himachalene(21.32%)-α-醇(7.71%)和长叶烯(6.74%)是EO的主要化合物。对雪松EO对两种病原真菌:镰刀菌(Fusarium culmorum)和灰葡萄孢(Botrytis cineria)的体外抗真菌活性进行了测试。最小抑制浓度(MIC)通过储备溶液的连续稀释来确定。经索氏、浸渍和超声提取的EOs对灰葡萄球菌具有重要的抑制作用(MIC=1.25mL/L)。然而,禾秆镰刀菌对所有测试的EOs都表现出抗性。这项研究的发现清楚地表明,EOs的挥发性成分可以根据提取而变化。方法。此外,抗真菌作用取决于EOs的化学成分,也取决于测试的淀粉。
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The effect of extraction methods on the yields, chemical composition and antifungal activity of sawdust Cedrus atlantica Manetti essential oils
The aim of this work is to determine the effect of the extraction method on the yield, the chemical composition, and the antifungal activity of cedarwood essential oils (EOs) from sawdust of Moroccan Cedrus atlantica (C. atlantica). EOs were extracted by different methods: hydrodistillation, soxhlet, maceration, and ultrasound. The chemical composition was determined using gas chromatography mass spectrometry (GC-MS) analysis. The yields of EOs were 5.60%, 11.68%, 4.82% and 9.33% for hydrodistillation, soxhlet, maceration and ultrasound, respectively. GC-MS revealed a diversity of chemical compounds depending on extraction methods. Indeed, the main compound of EOs obtained by soxhlet, maceration, and ultrasound was copalic acid methyl ester by a rate of 28.41%, 20.24%, and 24.17%, respectively. However, -himachalene (21.32%) followed by -himachalene (9.40%), β-Copaen-4α-ol (7.71%) and longifolene (6.74%) are the main compounds of EO extracted by hydrodistillation. The antifungal activity of cedarwood EO was tested in vitro on two pathogenic fungi: Fusarium culmorum (F. Culmorum) and Botrytis cineria (B. Cinerea). The minimum inhibitory concentration (MIC) was determined by successive dilutions of the stock solutions. The extracted EOs by soxhlet, maceration and ultrasound showed the important inhibitory effect against B. cinerea (MIC=1.25 mL/L). However, F. culmorum showed resistance towards all tested EOs. The finding of this study showed clearly that the volatile composition of EOs can be variable according to extraction. methods. Moreover, antifungal effects are depending on chemical composition of EOs but also to tested staris.
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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