R. Lemoui, S. Benyahia, Labib Noman, Ilham Bencherchar, F. Oke-Altuntas, K. Rebbas, S. Benayache, F. Benayache, I. Demirtaş
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Fraction T36 (IC<sub>50</sub><5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC<sub>50</sub><50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of <em>B. hispanica</em>.</p><p class=\"Abstract\"><strong>Video Clip of Methodology</strong>:</p><p class=\"Abstract\">Antiproliferative Assay: 4 min 31 sec <a href=\"https://www.youtube.com/v/vi_fS47fpY0&t=3s\">Full Screen</a> <a href=\"https://www.youtube.com/watch?v=vi_fS47fpY0&t=3s\">Alternate</a></p>","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":" ","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2018-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJP.V13I2.36133","citationCount":"7","resultStr":"{\"title\":\"Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria\",\"authors\":\"R. Lemoui, S. Benyahia, Labib Noman, Ilham Bencherchar, F. Oke-Altuntas, K. Rebbas, S. Benayache, F. Benayache, I. Demirtaş\",\"doi\":\"10.3329/BJP.V13I2.36133\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p class=\\\"Abstract\\\">The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of <em>Berberis hispanica</em>. Three phenolic compounds (tamarixetin, caffeic acid and rutin) were isolated from <em>B. hispanica</em>. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against <em>Klebsiella pneumonia</em> (15 mm at 100 mg/mL). Fraction T36 (IC<sub>50</sub><5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC<sub>50</sub><50 μg/mL) on HeLa cells. 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Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria
The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of Berberis hispanica. Three phenolic compounds (tamarixetin, caffeic acid and rutin) were isolated from B. hispanica. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against Klebsiella pneumonia (15 mm at 100 mg/mL). Fraction T36 (IC50<5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC50<50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of B. hispanica.
期刊介绍：
Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS).
A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements.
Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review.
Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.
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