报道细胞接种密度对癌症细胞系生长模式的影响

Q4 Pharmacology, Toxicology and Pharmaceutics Iranian Journal of Pharmaceutical Sciences Pub Date : 2017-04-01 DOI:10.22034/ijps.2017.31154
M. Nakhjavani, F. Shirazi
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引用次数: 2

摘要

癌细胞系的体外研究仍然是癌症研究的重要组成部分。研究人员考虑到处理不同细胞系的基本条件的重要性是非常重要的。据作者所知,本研究首次报道了播种密度对三种人类癌细胞系生长曲线的影响。这些细胞系包括人乳腺腺癌MCF-7细胞系、人卵巢癌A2780细胞系和人黑色素瘤A375细胞系。以4000、5000、15000、50000个细胞/cm2 4种不同的播种密度播种。细胞播种后,每天用台盼蓝法计数。这个实验做了三个重复。利用microsoftexcel构建细胞生长曲线,计算群体倍增时间(PDT)、滞后时间(LT)和饱和密度(SD)。结果表明,播种密度对生长曲线的总体格局有影响。在较低的播种密度下,生长曲线比在较高的播种密度下具有更预期的模式。此外,播种密度对土壤的低温无显著影响,但对土壤的干燥度和干燥度有一定影响。
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Reporting the Effect of Cell Seeding Density on Growth Pattern of Cancer Cell Lines
In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include human breast adenocarcinoma MCF-7 cell line, human ovary carcinoma A2780 cell line and human melanoma A375 cell line. The cell lines were seeded at 4 different seeding densities: 4000, 5000, 15000 and 50000 cells/cm2 of culture surface. Following seeding the cells and on daily basis, cell count was done with trypan blue assay. The experiment was done in triplicate. The growth curves of the cells were constructed using Microsoft Excel and population doubling time (PDT), lag time (LT) and saturation density (SD) were calculated. The results showed that the seeding density affects the general pattern of the growth curve. At lower seeding densities, the growth curves have a more expected pattern rather than in higher seeding densities. Also, seeding density does not affect the LT, while it has some effects on SD and PDT.
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来源期刊
Iranian Journal of Pharmaceutical Sciences
Iranian Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.50
自引率
0.00%
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0
期刊介绍: Iranian Journal of Pharmaceutical Sciences (IJPS) is an open access, internationally peer-reviewed journal that seeks to publish research articles in different pharmaceutical sciences subdivisions: pharmacology and toxicology, nanotechnology, pharmaceutics, natural products, biotechnology, pharmaceutical chemistry, clinical pharmacy and other pharmacy related topics. Each issue of the journal contents 16 outstanding research articles in area of pharmaceutical sciences plus an editorial written by the IJPS editors on one of the most up to date advances topics in pharmacy. All articles published by IJPS would be permanently accessible online freely without any subscription charges. Authors of the published articles have granted the right to use and disseminate their article to third parties.
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