elemi精油的抗群体感应和细胞毒性活性

A. Soyocak, A. Ak, E. Önem
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引用次数: 1

摘要

精油具有多种生物活性,如抗菌、抗氧化、增殖和抗炎。本研究旨在鉴定Elemi精油(EO)中的生物活性化合物,并测定EO的抗群体感应和细胞毒性活性。本研究采用气相色谱-质谱联用技术对EO的生物活性化合物进行了分析,并筛选了elemi对金黄色葡萄球菌ATCC 25923、耐甲氧西林金黄色葡萄菌ATCC 43300、大肠杆菌ATCC 25922、铜绿假单胞菌ATCC 27853、绿脓杆菌PAO1的抗菌活性。还研究了铜绿假单胞菌PAO1的抗生物膜活性和绿脓蛋白的产生。通过噻唑蓝-溴化四氮唑蓝(MTT)和中性红摄取(NR)法分析EO对成纤维细胞活力的影响。根据GC结果,EO的主要成分为柠檬烯(55%)。elemi精油的亚MIC分别抑制了43%和56%的生物膜形成和绿脓蛋白的产生。另一方面,EO对成纤维细胞系的线粒体和溶酶体活性也有急性影响。EO浓度作用24小时和48小时后,线粒体和溶酶体活性显著降低(p<0.05)。总之,EO对生物膜的形成和脓青蛋白的产生具有抑制活性,而且低剂量的油对成纤维细胞没有毒性作用。然而,与成纤维细胞系的溶酶体活性相比,更高剂量的EO对线粒体活性具有更大的细胞毒性作用。人们认为EO表现出这些活性是由于其含量中柠檬烯的量。
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Anti-quorum sensing and cytotoxic activity of elemi essential oil
Essential oils have several biological activities such as antimicrobial, antioxidant, proliferative, and anti-inflammatory. This study aimed identification of bioactive compounds found in Elemi essential oil (EO) and to determine the anti-quorum sensing and cytotoxic activities of EO. In this study, bioactive compounds of EO were analyzed using GC-MS, and the antibacterial activity of elemi was screened against Staphylococcus aureus ATCC 25923, Methicillin-Resistant Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PAO1. Anti-biofilm activity and pyocyanin production on P. aeruginosa PAO1 were also investigated. The effect of EO on cell viability was also analyzed by thiazolyl blue tetrazolium bromide (MTT) and neutral red uptake (NR) assay in fibroblast cells. According to GC results, the major component of EO was determined as limonene (55%). A sub-MIC of elemi essential oil inhibited biofilm formation and pyocyanin production by 43% and 56%, respectively. On the other hand, EO also had an acute effect on the mitochondrial and lysosomal activities of fibroblast cell lines. Mitochondrial and lysosomal activities were significantly decreased when EO concentrations were applied for 24 and 48 hours (p<0.05). In conclusion, EO has inhibitory activity on biofilm formation and pyocyanin production, and also the lower doses of oil have no toxic effects on fibroblast cells. However, higher doses of EO have more cytotoxic effects on mitochondrial activity rather than the lysosomal activity of fibroblast cell lines. It is thought that EO exhibits these activities due to the amount of limonene in its content. 
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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