亚历山大省西葫芦黄色花叶病毒对南瓜果实的自然感染

Journal of plant science and phytopathology Pub Date : 2020-04-20 DOI:10.29328/journal.jpsp.1001047

Abd El-Aziz Mahmoud Hamdy, Ahmed Eman Abd El tawab, Farag Azza Galal

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引用次数: 1

摘要

从亚历山大省Abees地区自然感染西葫芦果实中分离到一株西葫芦黄花叶病毒(ZYMV)。疾病症状表现为花叶状、黄化、起泡、脓肿症状。根据诊断宿主出现的症状和抗血清对黄瓜花叶黄瓜病毒(CMV)、西瓜花叶病毒2 (WMV-2)和ZYMV的血清学反应进行鉴定。ZYMV的南瓜果实分离物通过棉蚜、黑蚜和桃蚜以非宿存方式传播。用超离心和聚乙二醇分离纯化病毒。纯化的病毒具有典型的核蛋白紫外吸收光谱，A260/280和A280/260分别为1.1

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Natural infection of squash fruits (Cucurbita pepo) by Zucchini Yellow Mosaic potyvirus (ZYMV) in Alexandria governorate

An isolate of zucchini yellow mosaic virus (ZYMV) was obtained from naturally infected squash fruits were grown in Abees region, Alexandria governorate. Disease symptoms were Showing mosaic, yellowing and blistering and absis symptoms. The identiﬁ cation was based on the symptoms developed on diagnostic hosts and serological reactions with antisera to cucumber mosaic cucumovirus (CMV), watermelon mosaic potyvirus 2 (WMV-2) and ZYMV. Squash fruit isolate of ZYMV was transmitted by Aphis gossypii, aphis neri and Myzus persicae in non-persistent manner. The virus was puriﬁ ed by ultra-centrifugation and PEG. The puriﬁ ed virus had an ultraviolet absorption spectrum typical of a nucleoprotein with A260/280 and A280/260 being 1.1

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Journal of plant science and phytopathology

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