两系鸡热休克蛋白HSP70和HSP90的单核苷酸多态性鉴定

IF 3.5 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Annals of Agricultural Science Pub Date : 2020-12-01 DOI:10.1016/j.aoas.2020.07.002
A. Galal, Lamiaa M. Radwan
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引用次数: 6

摘要

气候变化影响到整个世界,导致高温,从而对家禽生产产生负面影响。因此,我们选择正式选育四代的丹达拉维(Dandarawi)和莱霍恩(Leghorn)两种鸡系,对热应激条件下产生热休克蛋白HSP70和HSP90的单核苷酸多态性(snp)进行检测,以获得更好的生长速率。在16周龄时,对丹达拉维和莱格霍恩雄性进行体重和身体测量。检测HSP70和HSP90基因的mRNA表达水平,并通过DNA测序检测这两个基因的多态性。结果表明,在热应激条件下饲养4代后,丹达拉维系和莱格霍恩系的鸡在体重和体尺上是可区分的。此外,与Leghorn系相比,Dandarawi系的HSP70和HSP90基因表达显著增加。这些结果表明,丹达拉维品种具有使其能够适应炎热气候条件的基因。因此,当选择高温增产时,基因表达量是相同选择条件下Leghorn菌株的两倍。丹达拉维鸡的耐热性优于来角鸡。在5号染色体52784621:52784671的HSP70外显子1区,由于T核苷酸被C取代,氨基酸为半胱氨酸而不是精氨酸,表明HSP70基因表达水平发生了改变。因此,我们建议使用HSP70基因外显子1 52784671和52784621区域的5号染色体作为耐热性评价的候选基因。
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Identification of single nucleotide polymorphism in heat shock protein HSP70 and HSP90 after four selection generations in two lines of chickens

Climatic changes have affected the entire world resulting in high temperatures, consequently leading to a negative impact on poultry production. Therefore, we conducted this study to detect the single nucleotide polymorphisms (SNPs), which is responsible for producing heat shock protein HSP70 and HSP90 in two selected lines of chicken, Dandarawi and Leghorn that were formally selected for four generations, for achieving a better growth rate under heat pressure. At age 16 weeks, body weight and body measurements were evaluated for Dandarawi and Leghorn males. mRNA levels of HSP70 and HSP90 genes were measured, and the polymorphism of these two genes was examined via DNA sequencing. Results showed that after four generations under heat stress conditions, the Dandarawi and Leghorn lines of chicken were distinguishable in terms of body weight and body measurements. Additionally, HSP70 and HSP90 a highly significant increase in gene expression for the Dandarawi line compared to the Leghorn line. These results indicate that the Dandarawi breed has genes that enable it to adapt under hot climate conditions. Hence, when selected to increase production under high temperatures, the gene expression is twice that of the Leghorn strain under the same conditions of selection. The Dandarawi chicken had better heat tolerance than the Leghorn chicken. The difference was observed on chromosome 5 in the region 52784621: 52784671 in exon 1 in HSP70 that was caused by the amino acid cysteine instead of arginine because of the substitution of T nucleotide with C, indicating the alteration in the gene expression level of HSPs. Therefore, we recommend using chromosome 5 in the region 52784621: 52784671 in exon 1 in HSP70 as a candidate gene in selection programs for evaluating heat stress tolerance.

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来源期刊
Annals of Agricultural Science
Annals of Agricultural Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
12.60
自引率
0.00%
发文量
18
审稿时长
33 days
期刊介绍: Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.
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