肠道病毒环境监测的比较研究:两相分离法与混合纤维素酯(MCE)膜过滤法

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Biosafety and Health Pub Date : 2023-06-01 DOI:10.1016/j.bsheal.2023.04.001
Ling Fang , Meizhong Chen , Shuangli Zhu , Wei Zhang , Dongmei Yan , Xiaolei Li , Shufen Huang , Caixia Li , Xue Guo , Hanri Zeng , Bixia Ke , Hui Li , Wenbo Xu , Changwen Ke , Xiaoling Deng , Yong Zhang , Huanying Zheng
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引用次数: 0

摘要

本研究旨在比较两相分离法和混合纤维素酯(MCE)膜过滤法检测污水中肠道病毒的灵敏度。2015年12月至2016年7月,在广州利德污水处理厂每月采集4份生活污水样本(1 L/份),每份污水样本分成2份(500 mL)。采用两相分离和MCE膜过滤的方法对污水样品进行浓缩,并将处理后的溶液接种于细胞中分离肠道病毒。对阳性分离株进行聚合酶链反应扩增、VP1测序和肠病毒分子分型。MCE膜过滤法对脊髓灰质炎病毒(PV)和非脊髓灰质炎肠道病毒(NPEV)的检出率高于两相分离法。McNemar检验显示PV、NPEV、1型Sabin-like (SL1)、2型Sabin-like (SL2)、3型Sabin-like (SL3)菌株的检出率均无统计学意义(P >0.05)。广东省PV和NPEV的检出率分别为53.13%和62.50%(20/32)。分离到27株pv,其中3株为1型疫苗,与1型Sabin菌株相比,VP1区有7个核苷酸替换。共分离NPEV 87株,鉴定出9种血清型,其中柯萨奇病毒B3 (CVB3)、埃可病毒6(E6)和埃可病毒11(E11)为优势毒株。使用MCE膜的过滤方法比两相分离更敏感,可以作为一种可靠、灵敏和经济的方法从污水中分离肠道病毒。
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A comparative study on environmental surveillance of enterovirus: Using a two-phase separation method and a filtration method with a mixed cellulose ester (MCE) membrane

This study aimed to compare the sensitivity of two-phase separation and the filtration method using a mixed cellulose ester (MCE) membrane to detect enteroviruses in sewage samples. From December 2015 to July 2016, four domestic sewage samples (1 L/sample) were collected monthly from the Guangzhou Liede Sewage Treatment Plant, and each sewage sample was divided into two aliquots (500 mL). The sewage sample was concentrated using the two-phase separation and the filtration method using an MCE membrane, and the treated solutions were inoculated into cells for enterovirus isolation. Polymerase chain reaction amplification, VP1 sequencing, and enterovirus molecular typing were performed on the positive isolates. The detection rates of poliovirus (PV) and non-polio enterovirus (NPEV) obtained using the filtration method using an MCE membrane were higher than those using the two-phase separation method. McNemar's test showed that the detection rates of PV, NPEV, type 1 Sabin-like (SL1), type 2 Sabin-like (SL2), and type 3 Sabin-like (SL3) strain were not statistically significant (P > 0.05). In Guangdong Province, China, the detection rates for PV and NPEV were 53.13% and 62.50% (20/32), respectively. Twenty-seven PVs were isolated, three highly variable strains of the type 1 vaccine, with seven nucleotide substitutions in the VP1 region, compared with the type 1 Sabin strains. Eighty-seven strains of NPEV were isolated and nine serotypes were identified, among which coxsackievirus B3 (CVB3), echovirus 6(E6), and echovirus 11(E11) were the dominant strains. The filtration method using an MCE membrane is more sensitive than two-phase separation and can be used as a robust, sensitive, and cost-effective method to isolate enteroviruses from sewage.

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来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
期刊最新文献
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