精油对草莓灰霉病的替代抑制作用

Camila Santos, Valéria Pohlmann, Fernanda Ludwig
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引用次数: 0

摘要

草莓(Fragaria x ananassa)是一种易腐烂的水果,采后寿命短,真菌病发病率高,尤其是灰葡萄孢。为了控制和预防,经常使用合成产品,这对食品和环境安全产生了负面影响。本研究的目的是评价精油在体外和体内对绿僵菌生长和控制的抑制潜力。采用4个重复的完全随机设计,对百里香(thyymus vulgares L.)、肉桂(Cinnamomum cassia L.)、柠檬草(Cymbopogon flexuosus L.)、薄荷(Mentha piperita L.)和茶树(Melaleuca alternifolia Maiden & Betche)的精油进行了测试,并采用蒸压水作为对照。通过分级评价真菌在体外的生长情况。对草莓果实进行了灰孢杆菌的存在、硬度和总可溶性固形物含量的评价。考察了灰霉菌的离体生长、灰霉菌侵染率、总可溶性固形物含量和草莓的质地。百里香精油和肉桂精油对真菌体外生长的抑制作用较弱,分别为0.32和0.92。草莓有症状的比例低于茶树(8.33%)、百里香(25%)、柠檬草(25%)和薄荷(26.66%)处理。百里香精油和肉桂精油在体外试验中对葡萄灰霉病有抑制作用,薄荷精油、百里香精油、茶树精油和柠檬草精油在草莓果实中有抑制作用。
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Alternative suppression of Botrytis cinerea in strawberry using essential oils
Strawberry (Fragaria x ananassa) is a perishable fruit with a short post-harvest life and a high incidence of fungal diseases, especially Botrytis cinerea. For control and prevention, synthetic products are often used which negatively influence in the food and environmental security. The present work was carried out with the objective of evaluating the inhibitory potential of essential oils in the growth and control of the B. cinerea in vitro and in vivo. The essential oils of thyme (Thymus vulgares L.), cinnamon (Cinnamomum cassia L.), lemongrass (Cymbopogon flexuosus L.), peppermint (Mentha piperita L.) and tea tree (Melaleuca alternifolia Maiden & Betche) were tested, in addition to control made of autoclaved water, using a completely randomized design with 4 replications. The fungus growth was evaluated in vitro through grading. Strawberry fruits were evaluated for the presence of B. cinerea, firmness and total soluble solids content. The growth of the B. cinerea in vitro, the percentage of gray mold infection, the total soluble solids content and the texture of strawberry were evaluated. The in vitro growth of the fungus received lower scores with the essential oils of thyme and cinnamon, with values of 0.32 and 0.92, respectively, with greater suppression. The percentage of strawberry with symptoms were lower than the treatments with tea tree (8,33%), thyme (25%), lemongrass (25%) and peppermint (26,66%). The essential oils of thyme and cinnamon have potential for use in the control of the Botrytis cinerea in in vitro test and the essential oils of peppermint, thyme, tea tree and lemongrass have potential for use in the suppression in strawberry fruits.
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