Isolation, Characterization and Quantitative Enumeration of Lactic Acid Bacteria from Human Faeces

Human Faeces (HF) is a solid waste material that is secreted as left amount after digestion of food inside the small intestine of the body. It contains large number of viruses, bacteria, fungi, actinomycetes, archaebacteria etc of which Lactic Acid Bacteria (LAB) are very common and play an important role in digestion and immunity. Hence, LAB were isolated and enumerated from HFusing standard protocol in the present study to find out the qualitative and quantitative distribution of LAB in gut microbiome. LAB were isolated using MRS agar medium under anaerobic conditions and was found that Lactobacillus lactis, L. Acidophilus, L. fermentum and Enterococcus faecium were the dominant species and the populations varied from 3.5 x 106 to 4.5 x 1010CFU/mL. It shows that good populations of LAB in gut microbiome survive under anaerobic conditions. LAB have great efficiency to resist against antibiotics. Such species of LAB should be commercialized and marketed at a global stage so that problems related to imbalance in gut microbiome can be solved.