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Standardization and Evaluation of Buffers: A One Step DNA Extraction Protocol from Microbial Cultures 缓冲液的标准化和评价:从微生物培养物中一步提取DNA的方案
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.6
Poornima Shyam
Bacteria are one of the most diverse organisms that exist. Every day labs are flooded with cultures to detect the type of microorganisms and their antibiotic resistance patterns to treat patients. However, this reporting takes 3 days. Molecular biology is a field that is associated with quicker reporting. This work is an attempt to make molecular testing easier and quicker to help with one step DNA extraction method. 3 different buffers were tested for DNA extraction from cultures. The result of the best buffer is represented in agarose gel electrophoresis. Efficient extraction of DNA from bacteria was seen. The first step to standardization of buffers for the one-step extraction of DNA from bacteria. The buffers need to be tested and standardized with a wider variety of samples of both gram-positive and gram-negative organisms and an efficient method of extraction from the primary samples instead of the culture plates has to be standardized. This is a pilot study to screen the buffers for their effectiveness.
细菌是现存的最多样化的生物之一。每天,实验室里都挤满了培养物,以检测微生物的类型及其抗生素耐药性模式,以治疗患者。然而,这个报告需要3天时间。分子生物学是一个与快速报告相关的领域。这项工作是一种尝试,使分子检测更容易和更快,有助于一步DNA提取方法。测试3种不同缓冲液从培养物中提取DNA。最佳缓冲液的结果用琼脂糖凝胶电泳表示。从细菌中有效地提取了DNA。第一步是标准化缓冲液,用于一步提取细菌DNA。缓冲液需要用更多种类的革兰氏阳性和革兰氏阴性生物样本进行测试和标准化,并且必须标准化从初级样本中提取而不是从培养板中提取的有效方法。这是一项筛选缓冲剂有效性的初步研究。
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引用次数: 0
Didactic Strategy for the Use of Virtual Itineraries in the Training of Graduates in Education. Geography 在教育专业毕业生培训中使用虚拟行程的教学策略。地理
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.7
Oliday Aguilar Espinosa
The training of teachers has always been a necessity of the planet aligned to the space and time where this process takes place. Future training of graduates in education, Geography who study geographic space need novel ways to appropriate these contexts and knowledge. The objective of this research was to propose a didactic strategy with the use of virtual didactic itineraries, in the teaching-learning process for the training of education, Geography in the first year of the career: Bachelor of Education. Geography, through the subject Introduction to Labor Practice, which contributed to deepen the knowledge about different landscapes and at the same time developed professional skills related to the study of the landscape. For the development of the research, information collection methods were used (participatory observation, document review, interview, expert criteria) and information processing methods (analysis – synthesis, inductive-demonstrative, logical history, descriptive statistics, modeling and analysis). The methods allowed to determine the needs of the students from the cognitive, educational and procedural point of view, regarding the teaching-learning process. The didactic strategy was implemented in practice, achieving very satisfactory results from the 2019-2022 academic years. It allows contributing to the development of professional pedagogical skills while deepening the knowledge of different landscapes in the students of the Degree in Education. Geography of the Faculty of Secondary Education of the Central University “Marta Abreu” of Las Villas, Villa Clara, Cuba.
对教师的培训一直是地球与这一进程发生的空间和时间相一致的必要条件。未来教育、地理专业的毕业生需要用新的方法来适应这些背景和知识。本研究的目的是提出一种教学策略,使用虚拟教学行程,在教育培训的教学过程中,地理在职业生涯的第一年:教育学士学位。地理,通过《劳动实践导论》这门课程,加深了对不同景观的认识,同时也培养了与景观研究相关的专业技能。为了开展研究,使用了信息收集方法(参与式观察、文献回顾、访谈、专家标准)和信息处理方法(分析-综合、归纳-论证、逻辑历史、描述性统计、建模与分析)。这些方法可以从认知、教育和程序的角度来确定学生在教与学过程中的需求。教学策略在实践中得到了实施,2019-2022学年取得了令人满意的成绩。它可以促进专业教学技能的发展,同时加深教育学位学生对不同景观的了解。古巴维拉克拉拉拉斯维拉斯的中央大学“Marta Abreu”中等教育学院地理。
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引用次数: 0
Predictive Modeling of COVID- 19 Confirmed Cases Using Regressive Objective Regression Methodology 基于回归客观回归方法的COVID- 19确诊病例预测建模
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.1
Fernando Martínez Fernández
The use of predictive models for the evolution of the pandemic is of great help in decision-making by the authorities. The fundamental objective of this work was to obtain through the Regressive Objective Regression, predictions of confirmed cases of COVID-19 in the Marta Abreu Teaching Polyclinic of the city of Santa Clara. In short-term modeling the model was significant at 19.7% with an error of 0.12. Variables dichotommics, saw tooth and saw tooth inverted and risk returned in 1.3, and 12 cases the trend is negative and not significant. We can conclude that a perfect result was obtained in the long term with the ROR methodology. The short-term ROR model depends on the cases of COVID-19 in the previous case, 3 cases back and 12 cases back without significant trend. The long-term model is perfect and depends on the cases of COVID-19 in 12 cases ago, with a negative trend.
使用疫情演变的预测模型对当局的决策有很大帮助。这项工作的基本目标是通过回归客观回归获得圣克拉拉市Marta Abreu教学综合诊所新冠肺炎确诊病例的预测。在短期建模中,该模型的显著性为19.7%,误差为0.12。变量二分法,锯齿和锯齿倒置,风险回归1.3,12例呈阴性且不显著。我们可以得出结论,从长远来看,ROR方法获得了完美的结果。短期ROR模型取决于前一个病例中的新冠肺炎病例、3个病例和12个病例,没有显著趋势。长期模型是完美的,取决于12例前新冠肺炎病例,呈负趋势。
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引用次数: 0
Avifaunal Abundance of Lumding Forest Reserve Area, Assam, India 印度阿萨姆邦朗丁森林保护区的鸟类丰度
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.5
J. Arjun
The solitary aim of our investigation was to explore the avian species richness in Lumding Forest Reserve area, Assam.The survey was cooperated by the team of Zoological Survey of India. After extended survey, 146 species of birds were spotted from Lumding Forest Reserve area, where birds from 17 different orders (Passeriformes, Columbiformes, Accipitriformes , Piciformes, Bucerotiformes ,Gruiformes, Apodiformes, Coraciiformes, Trogoniformes, Cuculiformes, Galliformes, Ciconiiformes, Suliformes , Pelecaniformes , Strigiformes, Anseriformes, Charadriiformes) and 76 families were put down. The bird species thus obtained were compared with the IUCN Red list from where interesting cum astonishing facts were acquired. The IUCN Red list informed us that, 92.51% bird species spotted were under Least Concern category, where as 3.4% were Near Threatened, 2.72% were Vulnerable, 0.68% were Critically Endangered and Endangered. We considered ourselves successful for obtaining such astouned statistics about the avian species plentitude of Lumding Forest Reserve, which will provoke other researchers to come forward and explore more about this place.
本调查的唯一目的是探讨阿萨姆邦卢丁森林保护区鸟类物种丰富度。这次调查是由印度动物调查小组合作进行的。经扩大调查,在卢顶森林保护区共发现鸟类146种,共记录鸟类17目(雀形目、柱形目、钩形目、钩形目、钩形目、叉形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目、钩形目)76科。由此获得的鸟类物种与IUCN红色名录进行了比较,从那里获得了有趣和惊人的事实。IUCN红色名录告诉我们,92.51%的鸟类属于最不受关注的类别,其中近受威胁的占3.4%,易受威胁的占2.72%,极度濒危和濒危的占0.68%。我们成功地获得了泸定森林保护区鸟类种类丰富的惊人统计数据,这将激发其他研究人员挺身而出,对这个地方进行更多的探索。
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引用次数: 0
Localization of NPY Immunoreactivity in the Proximal and Distal Intestinal Region of Teleost Fish, Notopterus 硬骨鱼(Notopterus)肠道近端和远端NPY免疫反应性的定位
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.8
T. Khadse
The purpose of the present study was to investigate the immunocytochemical aspects of the intestine of the teleost fish Notopterus notopterus. The distribution and relative frequency of the endocrine cells in the intestine were studied immunocytochemically using Streptavidin biotin peroxidase complex method. The aim was analyse the distribution and localization of immunoreactivity in the intestine of Notopterus notopterus against the antisera NPY. The samples were taken from the proximal and distal intestine. The NPY immunoreactive endocrine cells were found in the distal intestine at low frequencies than proximal intestine. These immunoreactive cells were distributed among more in mucosa epithelium and very low in the lamina propria and submucosa of intestine of N.notopterus.
本研究的目的是研究硬骨鱼Notopterus Notopterus肠道的免疫细胞化学方面。采用链霉亲和素-生物素-过氧化物酶复合物法对肠内内分泌细胞的分布和相对频率进行了免疫细胞化学研究。目的分析Notopterus Notopterus肠道对NPY抗血清免疫反应的分布和定位。样本取自近端和远端肠。远端肠中出现NPY免疫反应性内分泌细胞的频率低于近端肠。这些免疫反应细胞主要分布在粘膜上皮,而在肠固有层和肠黏膜下层的分布很少。
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引用次数: 0
Ecology of Biofouling Phytoplankton in Chinnamuttom Harbour Waters Southeast Coast of India 印度东南海岸chinnamumtom港水域浮游植物生物污染生态学研究
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.9
P. Nithya
Phytoplankton are the primary source of food, attributed most zooplankton communities and larval stages, and many meroplankton such as bivalves, crustaceans, and fishes. In the present study, phytoplankton samples were collected from surface water and substratum of the ship hull during the period of June 2015 to May 2016. Population density recorded from water and substratum of the ship hull ranged from 1.5e+4 to 3.4e+4 cells/L. Shannon-Wiener diversity index (H‛) of station I and station II ranged from 5.448 to 6.134 bits/ind. Simpson’s richness range was 0.973 to 0.984 at station I and station II. Pielou’s Evenness index (J’) was found to be from 0.950 to 0.979 and Chlorophyll ‘a’ range was 0.277 to 1.326 mg/m3 in the station I and station II. During this entire study period, 74 species of phytoplankton and 54 species of phytoplankton in station II have been recorded. Data of the present study show that significant differences occurred in over all percentage composition of phyhtoplankton species, both in in water and substratum. In water samples, recorded data showed that 85% were of Bacillariophyceae, 8 % of Chlorophyceae and, 7% of Dinophyceae. On the other hand, 75 % Bacillariophyceae, 7 % Chlorophyceae, 16% Dinophyceae and 2% Cyanophyceae were recorded in the substratum. The marine biofouling phytoplankton density, diversity, richness, evenness and chlorophyll’a’ were recorded in their maximum levels during summer season, and minimum were obtained in the monsoon. The diatoms were the predominant groups among the phytoplankton. Among the diatoms, species like Nitzschia longissima, Coscinodiscus centralis and Fragilaria sp. were record almost in all the seasons in the present study.
浮游植物是主要的食物来源,大多数浮游动物群落和幼虫阶段,以及许多浮游生物,如双壳类、甲壳类和鱼类。本研究于2015年6月至2016年5月采集了船体表层水和底层浮游植物样本。从水和船体基底记录的种群密度范围为1.5e+4至3.4e+4细胞/L。站1和站2的Shannon-Wiener分集指数H′变化范围为5.448 ~ 6.134 bit /ind。站1和站2 Simpson丰富度范围为0.973 ~ 0.984。I站和II站的Pielou均匀度指数(J′)在0.950 ~ 0.979之间,叶绿素a′在0.277 ~ 1.326 mg/m3之间。在整个研究期间,共记录浮游植物74种,II站浮游植物54种。本研究的数据表明,无论是在水中还是在基质中,浮游植物种类的总体百分比组成都存在显著差异。在水样中,记录数据显示硅藻门占85%,绿藻门占8%,藻门占7%。其中硅藻门75%,绿藻门7%,藻门16%,蓝藻门2%。海洋生物污染浮游植物的密度、多样性、丰富度、均匀度和叶绿素a值在夏季最高,在季风期最低。硅藻是浮游植物中的优势类群。在硅藻中,最长硅藻(Nitzschia longissima)、中央尾盘藻(Coscinodiscus centralis)和Fragilaria sp.几乎在所有季节都有记录。
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引用次数: 0
Adsorption of Phenol and Resorcinol on Parthenium Based Activated Carbon (Pac) in Basal Salt Medium: Equilibrium and Kinetics Parthenium基活性炭吸附苯酚和间苯二酚的平衡及动力学研究
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.4
Khushi Gandhi
The adsorption studies were carried out for the removal of Phenol and Resorcinol on Parthenium Based Activated Carbon (PAC) at 301 K and neutral pH ~7. To determine the adsorption equilibrium isotherms, the experiments were performed at the initial concentration of adsorbates with adsorbent doses from 1 g/l to 10 g/l. The experimental data were fitted to six isothermal models, and a nonlinear least square method has been used to estimate model parameters. It has been observed that for adsorption on PAC, Freundlich model was best fitted for the study and data was more closely related for the removal of both the pollutants from industrial wastewater. In this study, the adsorption kinetics were also studied in three distinct phases-rapid, medium & amp: slow for the time period till 12 hours. The parameter values found in this study shall be useful in designing and activated carbon adsorber for treatment of industrial wastewater at large scale. The present work shows that Parthenium based activated carbon (PAC) was an effective method for the adsorption of phenol and resorcinol and p-cresol from aqueous solution. The adsorption of phenol and its derivatives are in the following order: Phenol is adsorbed in maximum amount Resorcinol is comparatively adsorbed lesser than phenol.
研究了在301K、中性pH~7条件下Parthenium基活性炭(PAC)对苯酚和间苯二酚的吸附性能。为了确定吸附平衡等温线,在吸附剂剂量为1g/l至10g/l的吸附质的初始浓度下进行实验。将实验数据拟合到六个等温模型中,并使用非线性最小二乘法估计模型参数。已经观察到,对于在PAC上的吸附,Freundlich模型最适合该研究,并且数据与从工业废水中去除这两种污染物更密切相关。在本研究中,还研究了快速、中等和慢速三个不同阶段的吸附动力学,持续时间为12小时。本研究中发现的参数值将有助于设计用于大规模处理工业废水的活性炭吸附器。研究表明,Parthenium基活性炭(PAC)是从水溶液中吸附苯酚、间苯二酚和对甲酚的有效方法。苯酚及其衍生物的吸附顺序为:苯酚吸附量最大,间苯二酚的吸附量相对小于苯酚。
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引用次数: 0
Knowledge and management Assessment of Temporomandibular Joint (TMJ) Disorders Among Dental Interns at King Saud University 沙特国王大学牙科实习生颞下颌关节(TMJ)疾病的知识和管理评估
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.10
H. Albagieh
The temporomandibular joint (TMJ) complex is mainly composed of bone, cartilage, muscles, ligaments, and neurovascular channels that supply the tissues. The temporomandibular disorders (TMD) are collective term embracing a number of clinical problems that involve the masticatory muscles, the temporomandibular joints (TMJs) and associated structures, or both. The most common symptoms of temporomandibular joint disorders (TMDs) are pain, noises in the joint, limitation of mouth opening, or a combination of these. The objective of this study was to assess the knowledge and management of temporomandibular joint (TMJ) disorders among dental interns at King Saud University, Saudi Arabia. The sample size of this study consisted of 100 dental interns at King Saud University. Informed consent from the participants was obtained. The participants were informed that their responses and personal information would be kept confidential. A cross-sectional survey was prepared in English language using Google Forms. The survey contains 12 questions was distributed among the selected sample of dental interns at King Saud University. The average knowledge score of the dental interns on the knowledge regarding management of (TMJ) disorders was 4.04 (SD = 1.42). The maximum score obtained was 8 out of 8, the minimum was zero. that the majority of the dental interns (67%) had fair knowledge. The finding of the present study showed, there was a fair level of knowledge about (TMJ) disorders among dental interns. Many interns are unable to diagnose and treat patients suffering from temporomandibular joint disorders.
颞下颌关节(TMJ)复合体主要由骨、软骨、肌肉、韧带和供应组织的神经血管通道组成。颞下颌关节疾病(TMD)是包括咀嚼肌肉、颞下颌关节(TMJs)和相关结构,或两者均涉及的一系列临床问题的总称。颞下颌关节紊乱(TMDs)最常见的症状是疼痛、关节噪音、张嘴受限或这些症状的组合。本研究的目的是评估沙特阿拉伯沙特国王大学牙科实习生对颞下颌关节(TMJ)疾病的知识和管理。本研究的样本量包括100名来自沙特国王大学的牙科实习生。获得了参与者的知情同意。参与者被告知,他们的回答和个人信息将被保密。采用谷歌表格,用英语进行横断面调查。该调查包含12个问题,在沙特国王大学的牙科实习生中进行了抽样调查。牙科实习生TMJ疾病管理知识平均得分为4.04分(SD = 1.42)。满分为8分,满分为8分,满分为0分。大多数牙科实习生(67%)有公平的知识。本研究结果显示,牙科实习医师对颞颌关节疾病有相当程度的了解。许多实习生无法诊断和治疗患有颞下颌关节疾病的患者。
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引用次数: 0
BMI for Age Percentile As Criteria for Assessment of Obesity in Children and Adolescents 年龄百分比BMI作为儿童和青少年肥胖评估标准
Pub Date : 2023-06-25 DOI: 10.21786/bbrc/16.2.3
M. Dewan
The most useful population-level measure of overweight and obesity is BMI. This measure is same for men and women of all ages. But it should not be thought to be as final factor to calculate the same body fat percentage in different individuals. Various proposed reference values for overweight and obesity exist for BMI, but the combined effect of BMI and BMI for age percentile recommendations is scarce. A total 2048 children participated in the epidemiological survey. According to Body Mass Index in the study, 66.26% subjects were found to be malnourished in the total population. The degree of obesity had been observed as Pre-obese 3.81% and Obese-I 3.81% and Obese-II were 0.88%. Pre-obese, obese-I and obese-II boys were more than the girls. According to BMI-for-age percentile, overall, 6.20% of children and adolescents were at risk of overweight and 4.39% were overweight during health survey while 24.80% are underweight.
衡量超重和肥胖的最有用的人群水平是BMI。这项措施对所有年龄段的男性和女性都是一样的。但这不应该被认为是计算不同个体相同体脂百分比的最终因素。BMI存在各种超重和肥胖的参考值,但BMI和BMI对年龄百分位数建议的综合影响很少。共有2048名儿童参加了流行病学调查。根据研究中的体重指数,总人口中有66.26%的受试者营养不良。肥胖程度为肥胖前期3.81%,肥胖一期3.81%,肥胖症二期0.88%。肥胖前期、肥胖一期和肥胖二期男孩多于女孩。根据年龄百分位数的BMI,总体而言,在健康调查中,6.20%的儿童和青少年有超重的风险,4.39%的儿童和少年超重,24.80%的儿童和少女体重不足。
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引用次数: 0
Potential Application of the Objective Regression Regressive Methodology 客观回归回归方法论的潜在应用
Pub Date : 2023-03-25 DOI: 10.21786/bbrc/16.1.9
R. F. Duarte
The possibility of having a methodology that allows the modeling and prediction, in the short, medium and long term, of biological, social and natural disaster processes and/or phenomena is something great. The objective of the research consisted in demonstrating the potentialities and real capacity of application of the methodology of the Regressive Objective Regression (ROR) in the different fields and branches of scientific research. In the ORR methodology, in a first step, dichotomous variables DS, DI and NoC are created. Then, the module corresponding to the Regression analysis of the SPSS statistical package (ENTER method) is executed, where the predicted variable and the ERROR are obtained; subsequently, the autocorrelograms of the ERROR variable are obtained, paying attention to the maximum of the significant partial autocorrelations, and the new variables are calculated according to the significant Lag of the PACF. Finally, these regressed variables are included in the new regression in a process of successive approximations until a white noise is obtained. Wide possibilities of modeling and forecasting in the short, medium and long term, which go beyond the modeling of infectious entities of parasitic and viral etiology, Acute Respiratory Infections, Acute Bronchial Asthma crises, forecasting of extreme meteorological disturbances, prediction of latitude and longitude of earthquakes, modeling of climatic variables, and even the own electric consumption of a municipality, province and nation. The ROR methodology has demonstrated potential and real capabilities of application in dissimilar fields and branches of science, so it is a novel contribution to the science of modeling and forecasting of variables to know the future, as well as the impact that different variables contribute to an event or phenomenon, and being universal, it can be applied anywhere in the universe.
有一种方法可以在短期、中期和长期内对生物、社会和自然灾害过程和/或现象进行建模和预测,这是一种巨大的可能性。研究的目的是展示回归客观回归方法在不同科学研究领域和分支中应用的潜力和实际能力。在ORR方法中,在第一步中,创建了二分变量DS、DI和NoC。然后,执行SPSS统计包的回归分析(ENTER方法)对应的模块,得到预测变量和ERROR;随后,注意显著部分自相关的最大值,获得ERROR变量的自相关图,并根据PACF的显著滞后计算新变量。最后,这些回归变量在逐次逼近的过程中被包括在新的回归中,直到获得白噪声。短期、中期和长期建模和预测的可能性很大,超出了寄生虫和病毒病因的传染实体建模、急性呼吸道感染、急性支气管哮喘危机、极端气象干扰预测、地震经纬度预测、气候变量建模、,甚至是一个市、省和国家自己的电力消耗。ROR方法已经证明了在不同领域和科学分支中应用的潜力和实际能力,因此它是对变量建模和预测科学的一项新贡献,以了解未来,以及不同变量对事件或现象的影响,并且具有普遍性,可以应用于宇宙中的任何地方。
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引用次数: 0
期刊
Bioscience Biotechnology Research Communications
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