植物凝集素基因与蛋白的计算机比较分析

IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-06-09 DOI:10.47836/pjtas.46.3.06
Fathiya Khairiya, F. Dwivany, S. Suhandono, Sofia Safitri Hessel, I. Zainuddin, T. Tallei
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引用次数: 0

摘要

凝集素是一个能够识别和结合特定碳水化合物的蛋白质家族。植物凝集素在植物防御中发挥着多种作用,可作为杀虫、抗菌、抗真菌和抗病毒药物。这项研究使用计算机方法比较了15种植物凝集素之间的基因、蛋白质和碳水化合物结合基序。对Artocarpus hypargyreus Hance、Hordeum vulgare var.Betzes、Triticum aestivum L.cv.Marshall、Galanthus nivalis L.、Allium sativum L.、Phaseolus vulgaris、Lens culinaris subsp。绒毛状、Robinia pseudoacacia、Glycine max、Cicer arietinum、Pisum sativum、Canavalia ensiformis、Amaranthus caudatus、Amaranthaus hypochodiacus和Musa acuminata subsp。软化症来自国家生物技术信息中心和香蕉基因组中心。基因比较结果显示,15个植物凝集素基因具有不同的特征,其中A.hypargyreus的凝集素基因最短,G.max的凝集素基因长度最长。总的来说,这15个植物凝集素基因有1-3个外显子。结构域预测显示存在五个结构域:jacalin、chitin_bind_1、B_凝集素、豆类凝集素和凝集素。此外,与文献中的一致基序相比,有2个来自jacalin结构域的蛋白质序列、2个来自chitin _ bind_。从这项研究中获得的数据以前没有报道过,可以用于未来通过合成生物学方法生产凝集素蛋白。这种方法将使科学家能够使用异源系统获得用于凝集素生产的植物生物制品,即使没有植物样本。
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In silico Comparative Analysis of Gene and Protein of Plant Lectins
Lectins are a family of proteins that can recognize and bind specific carbohydrates. Plant lectins play various roles in plant defense and can be utilized as insecticidal, antibacterial, antifungal, and antiviral agents. This study compares genes, proteins, and carbohydrate-binding motifs between 15 plant lectins using in silico methods. The lectin genes of Artocarpus hypargyreus Hance, Hordeum vulgare var. Betzes, Triticum aestivum L. cv. Marshall, Galanthus nivalis L., Allium sativum L., Phaseolus vulgaris, Lens culinaris subsp. tomentosus, Robinia pseudoacacia, Glycine max, Cicer arietinum, Pisum sativum, Canavalia ensiformis, Amaranthus caudatus, Amaranthus hypochondriacus, and Musa acuminata subsp. malaccensis were obtained from National Center for Biotechnology Information and Banana Genome Hub. The gene comparison results revealed different characteristics of the 15 plant lectin genes, with A. hypargyreus having the shortest lectin gene and G. max having the longest. Overall, the 15 plant lectin genes have 1–3 exons. Domain predictions revealed the presence of five domains: jacalin, chitin_bind_1, B_lectin, legume lectin, and agglutinin. Furthermore, there were 2 protein sequences from the jacalin domain, 2 protein sequences from the chitin_bind_I domain, 2 protein sequences from the B_lectin domain, and 4 protein sequences from the legume lectin domains that have complete carbohydrate-binding motifs compared to consensus motifs from literature. The data obtained from this study has not been previously reported and can be utilized for future lectin protein production with synthetic biology approaches. This method will allow scientists to obtain plant bioparts for lectin production using a heterologous system, even without plant samples.
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来源期刊
Pertanika Journal of Tropical Agricultural Science
Pertanika Journal of Tropical Agricultural Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.10
自引率
16.70%
发文量
64
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