LncRNA磷酸酯酶和Tensin同源物诱导的激酶1-AS通过海绵miR-98-5p促进胰岛素样生长因子1受体表达并促进膀胱癌症进展

Shunping Wang, DanPing Cheng, Bin Zheng
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摘要

背景:LncRNA PINK1-AS是一种已确定的癌症关键修饰因子,但其在膀胱癌症(BC)中的生物学功能尚不清楚。目前的工作试图探索PINK1-AS在BC中的作用和机制。方法:切除50对BC组织和匹配的癌旁正常组织,分析PINK1-AS、miR-98-5p和IGF1R的表达。以T24细胞为基础,通过CCK-8、流式细胞术和Transwell测定法分别评估其增殖、凋亡、侵袭和迁移活性。RIP和双荧光素酶报告基因分析验证了基因之间的结合关系。结果:PINK1-AS在BC组织中的表达异常高,并与BC患者的TNM分期和淋巴结转移有关。PINK1-AS敲低可延缓BC的恶性进展。过表达PINK1-AS具有相反的效果。沉默或促进PINK1-AS对BC的影响分别通过IGF1R和miR-98-5p的过表达而减轻。PINK1-AS与miR-98-5p竞争性结合以介导IGF1R的表达。结论:靶向异常过表达的lncRNA PINK1-AS,可以释放miR-98-5p对IGF1R的抑制作用,从而促进BC恶性肿瘤的发生。PINK1-AS/miR-98-5p/IGF1R轴可以用作BC的潜在治疗靶点。
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LncRNA Phosphatase and Tensin Homolog Induced Kinase 1-AS Promotes Insulin Like Growth Factor 1 Receptor Expression Through Sponge miR-98-5p and Contributes to Bladder Cancer Progression
Background: LncRNA PINK1-AS is an identified key modifier in cancers, but its biological function in bladder cancer (BC) remains unclear. The current work tried to explore the function and mechanism of PINK1-AS in BC. Methods: Fifty-five pairs of BC tissue and matched para-cancer normal tissue were excised to analyze PINK1-AS, miR-98-5p, and IGF1R expression. Based on T24 cells, the proliferative, apoptotic, invasive, and migratory activities were evaluated by CCK-8, flow cytometry, and Transwell assay correspondingly. RIP and dual luciferase reporter assays verified binding relationships between genes. Results: PINK1-AS expression was abnormally high in BC tissues, and was associated with TNM staging and lymph node metastasis in BC patients. PINK1-AS knockdown delayed the malignant progression in BC. Overexpressing PINK1-AS had the opposite effect. The impacts of silencing or promoting PINK1-AS on BC were mitigated by overexpression of IGF1R and miR-98-5p, respectively. PINK1-AS was competitively bound to miR-98-5p to mediate IGF1R expression. Conclusion: Targeting the abnormally overexpressed lncRNA, PINK1-AS, can release the inhibition of IGF1R by miR-98-5p, thereby promoting BC malignancy. PINK1-AS/miR-98-5p/IGF1R axis can be used as a potential therapeutic target for BC.
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