非瑟酮对氟西汀所致成年雄性白化大鼠肝脏改变的可能改善作用:组织学、免疫组织化学和生化研究

Q3 Medicine Journal of Microscopy and Ultrastructure Pub Date : 2023-02-07 eCollection Date: 2023-07-01 DOI:10.4103/jmau.jmau_84_22
Dina Fouad El Shaer, Hend Ibrahim Abd El Halim
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引用次数: 0

摘要

背景:氟西汀(FLX)是一种选择性5 -羟色胺再摄取抑制剂,被广泛用于治疗包括抑郁症在内的神经精神疾病,但大剂量会引起一些不良反应。非瑟酮(FIS)是一种存在于蔬菜和水果中的生物活性类黄酮,具有抗氧化、抗炎和抗癌作用。目的:探讨FIS对成年雄性白化大鼠FLX所致肝脏改变的可能改善作用。材料与方法:将48只大鼠分为4组,ⅰ组为对照组,ⅱ组口服FIS (100mg /kg/d),ⅲ组口服FLX (10mg /kg/d),ⅳ组同时给予FLX和FIS,剂量和方式与ⅱ组和ⅲ组相同。获得血液和肝脏样本并准备用于组织学,免疫组织化学和生化研究。结果:FLX组大鼠肝脏结构紊乱,肝细胞胞浆空泡化,炎性细胞浸润,血液外渗,血管充血,caspase-3、诱导型一氧化氮合酶面积百分比显著增加,胶质纤维酸性蛋白表达细胞数量显著增加,周期性酸-希夫染色面积百分比显著降低。此外,FLX显著提高了血清中天冬氨酸转氨酶和丙氨酸转氨酶水平。此外,FLX增加丙二醛水平,降低肝组织超氧化物歧化酶和谷胱甘肽(GSH)过氧化物酶,降低GSH水平。同时使用FIS可改善这些改变。结论:FIS可改善FLX诱导的成年雄性白化大鼠肝脏组织、免疫组织化学和生化改变。
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The Possible Ameliorating Role of Fisetin on Hepatic Changes Induced by Fluoxetine in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study.

Background: Fluoxetine (FLX) is one of the selective serotonin reuptake inhibitors, it is widely used to treat neuropsychiatric disorders including depression, but high doses can cause several adverse effects. Fisetin (FIS), a bioactive flavonoid presents in vegetables and fruits, has antioxidant, anti-inflammatory, and anticancer effects.

Aim: To evaluate the possible ameliorating effect of FIS on the hepatic alterations induced by FLX in adult male albino rats.

Materials and methods: Our study was done, for 3-weeks, on 48 rats that were divided into four groups: Group I (control), Group II received FIS orally (100 mg/kg/day), Group III received FLX orally (10 mg/kg/day), and Group IV concomitantly received FLX and FIS at the same dose and manner of groups II and III. Blood and liver samples were obtained and prepared for histological, immunohistochemical, and biochemical studies.

Results: FLX group revealed disturbed liver architecture, hepatocytes with vacuolated cytoplasm, inflammatory cellular infiltration, blood extravasation, and congestion of blood vessels in addition to, a significant increase in the area percentage of caspase-3, inducible nitric oxide synthase and the number of glial fibrillary acidic protein-expressing cells as well as a significant decrease in the area percentage of periodic acid-Schiff stain. Moreover, FLX significantly increased aspartate-aminotransferase and alanine-aminotransferase levels in the serum. In addition, FLX increased malondialdehyde level and decreased superoxide dismutase, glutathione (GSH) peroxidase, and reduced GSH levels in liver tissue. The concomitant administration of FIS ameliorated these alterations.

Conclusions: Administration of FIS ameliorated the histological, immunohistochemical, and biochemical alterations induced by FLX in the liver of adult male albino rats.

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