番木瓜(Carica木瓜L.)叶正己烷和乙醇提取物作为小葱害虫(甜菜夜蛾Hübner)天然杀虫剂的效果

Mery Puspita Sari, L. Nugroho, S. Sukirno
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引用次数: 0

摘要

葱是具有较高销售价值和消费水平的农产品之一。然而,众所周知,小葱的生产力容易受到破坏植物的生物的影响,例如小葱毛虫(夜蛾)。目前,大多数青葱种植户使用合成杀虫剂控制甜菜。合成杀虫剂有毒,长期使用会引起对害虫的抵抗力和环境污染。木瓜是一种具有植物杀虫剂潜力的天然成分。本研究旨在确定木瓜叶的正己烷和乙醇提取物的有效性和浓度,这两种提取物对毒蜥2龄幼虫的毒性和摄食能力的抑制最为有效。提取物通过正己烷(非极性)和乙醇(极性)溶剂的逐渐浸渍获得。用薄层色谱法测定木瓜叶中次生代谢产物的含量。结果表明,乙醇提取物对幼虫的毒性作用最强,最高可达96.67%。同时,正己烷提取物对幼虫取食的抑制作用大于乙醇提取物,最小取食面积为2,27mm。对幼虫的毒力和取食抑制作用最有效的浓度为3%。乙醇提取物的LC50值为0.0207%,正己烷提取物为0.0459%。已知这两种提取物都含有化合物基团,即单宁、萜类化合物、黄酮类化合物和生物碱。
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Effectiveness of n-hexane and ethanol extract of papaya (Carica papaya L.) leaves as shallot pest (Spodoptera exigua Hübner) natural insecticide
One of the agricultural commodities that has a high selling value and consumption level is shallot (Alium ascalonicum). However, the productivity of shallots is known to be susceptible to plant-destroying organisms, such as the shallot caterpillar (Spodoptera exigua). Currently, most shallot farmers control S. exigua using synthetic insecticides. Synthetic insecticides are poisonous and use for a long time  period will cause resistance to pests and environmental pollution. One of the natural ingredients that has the potential as a botanical insecticide is papaya (Carica papaya). This study aimed to determine the effectiveness and concentration of n-hexane and ethanolic extracts of papaya leaves which are the most effective against toxicity and inhibition of the feeding power of S. exigua second instar larvae. The extract was obtained by gradual maceration of n-hexane (non polar) and ethanol (polar) solvents. Test the secondary metabolite content of papaya leaves using Thin Layer Chromatography (TLC). The results showed that the ethanol extract was the most effective extract in causing toxicity to larvae with the highest percentage of 96.67%. Meanwhile, n-hexane extract was more effective in inhibiting larval feeding than ethanol extract with the lowest feeding area of 2,27 mm. The most effective concentration of against toxicity and feeding inhibition of larvae is concentration 3%. The LC50 value of the ethanol extract was 0.0207% and the n-hexane extract was 0.0459%. Both extracts are known to contain compound groups, namely tannins, terpenoids, flavonoids, and alkaloids.
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