M. Ascunce, R. V. Vander Meer, Satya Chinta, Cassandra S. Ogura-Yamada, D. Oishi
{"title":"夏威夷茧蜂的遗传和化学特征(膜翅目:蚁科)","authors":"M. Ascunce, R. V. Vander Meer, Satya Chinta, Cassandra S. Ogura-Yamada, D. Oishi","doi":"10.1653/024.105.0114","DOIUrl":null,"url":null,"abstract":"Summary Ants intercepted by the Hawaii Department of Agriculture in 2018 and 2019 identified as Solenopsis spp., and possibly S. invicta, were submitted to the United States Department of Agriculture-Agricultural Research Service-Center for Medical, Agricultural and Veterinary Entomology, Imported Fire Ant and Household Insects Research Unit for further taxonomic assessment using genetic and chemical methods. The 2018 sample consisted of 1 worker ant, and the 2019 sample consisted of 3 worker ants. Thoraces were used for genetic assessment and gasters were used for venom analyses. It was determined readily from genetic and chemical analyses that the intercepted ants were not S. invicta or S. richteri. Mitochondrial COX1 gene sequences were compared with previously published data from Solenopsis species. The closest match for the 2018 sample was S. xyloni, which is native to the shipment's origin (San Diego, California, USA). The 2 major alkaloids found were characteristic of the S. geminata species group. In addition, the alkaloid ratio closely matched that of S. xyloni. Therefore, the alkaloid analysis of the 2018 intercepted ant concurs with the genetic data that the ant was an S. xyloni worker. The COX1 gene sequences of the 2019 interceptions most closely matched S. geminata. The corresponding venom alkaloid profile also eliminated S. invicta and S. richteri as possibilities. However, 1 major alkaloid was unusual (Fig. 2) and normally is found only in trace amounts. We suggest the 2019 specimens are an unusual variant of S. geminata.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic and Chemical Profiling of Solenopsis spp. (Hymenoptera: Formicidae) Intercepted in Hawaii\",\"authors\":\"M. Ascunce, R. V. Vander Meer, Satya Chinta, Cassandra S. Ogura-Yamada, D. Oishi\",\"doi\":\"10.1653/024.105.0114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary Ants intercepted by the Hawaii Department of Agriculture in 2018 and 2019 identified as Solenopsis spp., and possibly S. invicta, were submitted to the United States Department of Agriculture-Agricultural Research Service-Center for Medical, Agricultural and Veterinary Entomology, Imported Fire Ant and Household Insects Research Unit for further taxonomic assessment using genetic and chemical methods. The 2018 sample consisted of 1 worker ant, and the 2019 sample consisted of 3 worker ants. Thoraces were used for genetic assessment and gasters were used for venom analyses. It was determined readily from genetic and chemical analyses that the intercepted ants were not S. invicta or S. richteri. Mitochondrial COX1 gene sequences were compared with previously published data from Solenopsis species. The closest match for the 2018 sample was S. xyloni, which is native to the shipment's origin (San Diego, California, USA). The 2 major alkaloids found were characteristic of the S. geminata species group. In addition, the alkaloid ratio closely matched that of S. xyloni. Therefore, the alkaloid analysis of the 2018 intercepted ant concurs with the genetic data that the ant was an S. xyloni worker. The COX1 gene sequences of the 2019 interceptions most closely matched S. geminata. The corresponding venom alkaloid profile also eliminated S. invicta and S. richteri as possibilities. However, 1 major alkaloid was unusual (Fig. 2) and normally is found only in trace amounts. We suggest the 2019 specimens are an unusual variant of S. geminata.\",\"PeriodicalId\":12297,\"journal\":{\"name\":\"Florida Entomologist\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2022-04-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Florida Entomologist\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1653/024.105.0114\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Florida Entomologist","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1653/024.105.0114","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Genetic and Chemical Profiling of Solenopsis spp. (Hymenoptera: Formicidae) Intercepted in Hawaii
Summary Ants intercepted by the Hawaii Department of Agriculture in 2018 and 2019 identified as Solenopsis spp., and possibly S. invicta, were submitted to the United States Department of Agriculture-Agricultural Research Service-Center for Medical, Agricultural and Veterinary Entomology, Imported Fire Ant and Household Insects Research Unit for further taxonomic assessment using genetic and chemical methods. The 2018 sample consisted of 1 worker ant, and the 2019 sample consisted of 3 worker ants. Thoraces were used for genetic assessment and gasters were used for venom analyses. It was determined readily from genetic and chemical analyses that the intercepted ants were not S. invicta or S. richteri. Mitochondrial COX1 gene sequences were compared with previously published data from Solenopsis species. The closest match for the 2018 sample was S. xyloni, which is native to the shipment's origin (San Diego, California, USA). The 2 major alkaloids found were characteristic of the S. geminata species group. In addition, the alkaloid ratio closely matched that of S. xyloni. Therefore, the alkaloid analysis of the 2018 intercepted ant concurs with the genetic data that the ant was an S. xyloni worker. The COX1 gene sequences of the 2019 interceptions most closely matched S. geminata. The corresponding venom alkaloid profile also eliminated S. invicta and S. richteri as possibilities. However, 1 major alkaloid was unusual (Fig. 2) and normally is found only in trace amounts. We suggest the 2019 specimens are an unusual variant of S. geminata.
期刊介绍:
Florida Entomologist is the official journal of the Florida Entomological Society. Volumes 1-3 were published under the name The Florida Buggist. The Florida Entomological Society still produces the traditionally printed version of Florida Entomologist, but you can also view, search, or print any article published since June 1917 by accessing online files. Web access is made possible by the Society’s electronic publication project begun in 1993