负载双金属金-银纳米粒子的新型生物粘附壳聚糖膜的设计及其抗生物膜和伤口愈合活性

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL Biomedical materials Pub Date : 2023-02-02 DOI:10.1088/1748-605X/acb89b
C. Singh, A. K. Mehata, V. ., P. Tiwari, Aseem Setia, Ankit Malik, Sanjeev K Singh, Rashmi M. Tilak, M. S. Muthu
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引用次数: 1

摘要

微生物感染和抗生素耐药性是全世界发病率和死亡率的主要原因之一。采用种子生长合成技术制备了双金属壳聚糖(CS)包覆的金-银纳米粒子(CS- auag - nps)。采用Box-Behnken设计,对纳米颗粒粒径(PS)、ζ电位(ZP)和抗菌活性进行了3个层次、3个因素的优化。制备的CS-AuAg-NPs是多分散的,平均水动力PS范围为55 ~ 289 nm, ZP范围为+8.53 mV ~ +38.6 mV。优化后的CS-AuAg-NPs对耐多药金黄色葡萄球菌ATCC 25923 (MDR AT)的最小抑菌浓度为1.625±0.68µg ml - 1,对耐多药金黄色葡萄球菌临床分离株MDR1695 (MDR CI)的最小抑菌浓度为3.25±0.93µg ml - 1,对耐多药金黄色葡萄球菌ATCC 25923 (MDR AT)的最小杀菌浓度为3.25±0.74µg ml - 1。与克林霉素标准相比,CS-AuAg-NPs对耐多药AT和耐多药CI更有效。临床分离菌株的活/死试验显示,与对照组相比,12小时内细菌细胞显著减少约67.52倍。溶血研究表明,CS-AuAg-NPs无溶血作用,应用于伤口更安全。此外,CS- auag - nps分布在CS膜中,在小鼠模型中,7 d后伤口恢复率为87%。因此,我们得出结论,CS-AuAg-NPs对耐多药细菌更安全,更有效,并且能够在感染伤口中进行皮肤再生。
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Design of novel bioadhesive chitosan film loaded with bimetallic gold-silver nanoparticles for antibiofilm and wound healing activity
Microbial infections and antibiotic resistance are among the leading causes of morbidity and mortality worldwide. The bimetallic chitosan (CS)-capped gold-silver nanoparticles (CS-AuAg-NPs) were prepared by the seeded growth synthesis technique. The nanoparticles were optimized for particle size (PS), zeta potential (ZP) and antibacterial activity by Box–Behnken design at three levels and three factors. The developed CS-AuAg-NPs were polydispersed with mean hydrodynamic PS in the range of 55 – 289 nm and ZP ranges from +8.53 mV to +38.6 mV. The optimized CS-AuAg-NPs found to have a minimum inhibitory concentration and minimal bactericidal concentration of 1.625 ± 0.68 and 3.25 ± 0.74 µg ml−1 towards multidrug resistant (MDR) Staphylococcus aureus ATCC 25923 (MDR AT) and 3.25 ± 0.93 and 3.25 ± 0.86 µg ml−1 towards MDR S. aureus clinical isolate MDR1695 (MDR CI) strain, respectively. The CS-AuAg-NPs were much more effective against MDR AT and MDR CI compared to clindamycin standard. The live/dead assay of clinical isolates strain demonstrated significant reduction of bacterial cells ∼67.52 folds compared to control group in 12 h. The hemolysis study suggested that CS-AuAg-NPs were non-hemolytic and safer for application in the wound. Furthermore, CS-AuAg-NPs were distributed in the CS film, which showed 87% wound recovery after 7 d in mice model. Hence, we concluded that CS-AuAg-NPs was safer and more effective against MDR bacteria and capable of skin regeneration in the infected wound.
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来源期刊
Biomedical materials
Biomedical materials 工程技术-材料科学:生物材料
CiteScore
6.70
自引率
7.50%
发文量
294
审稿时长
3 months
期刊介绍: The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters
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