In Vitro Callus Induction from Immature Fruits of Elaeocarpus ganitrus: A Tissue Culture Approach

In the tissue culture investigations for tree species, the growth medium with a perfect concentration of growth regulators and other components has a crucial role in the in vitro callus initiation from explants material. The present study has highlighted the effects of various concentrations of plant growth regulators on callus initiation from immature fruits of Elaeocarpus ganitrus in Murashige & Skoog (MS) medium. The immature fruits were collected as explants sources during fruiting season and cultured on 1/2, 3/4 and full-strength MS medium. In this study, 1.8mgL-1 of 2, 4-D, and full-strength MS medium was observed best for callus initiation and further supported for the multiplication from immature fruits of E. ganitrus. The callus initiation from immature fruits of E. ganitrus was the crucial step towards the conservation study of this tree species. This study can be helpful for tissue culture investigations concerning callus development from immature fruit explants material of several other species of Elaeocarpus and even for the other endangered tree species.