四种不同分子量麦冬多糖的结构特征及α-葡萄糖苷酶抑制活性

IF 4.8 3区化学 Q1 CHEMISTRY, MEDICINAL Natural Products and Bioprospecting Pub Date : 2023-06-06 DOI:10.1007/s13659-023-00384-1

Luan Wen, Zhou-Wei Wu, Li-Wu Lin, Abdulbaset Al-Romaima, Xing-Rong Peng, Ming-Hua Qiu

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引用次数: 1

摘要

从枸杞中分离得到四种多糖(MCPa、MCPb、MCPc、MCPd)。通过化学和仪器方法对其结构进行了表征，包括总糖、醛酸和蛋白质含量测定、紫外、红外和核磁共振光谱、单糖组成测定和甲基化分析。4种多糖是一组分子量在3.12 ~ 14.4 kDa之间的葡聚糖，它们共享一个相似的主链，由(1→4)-葡萄糖键组成，分支分别连接C-3和C-6。此外，生物活性测定表明，MCPs对α-葡萄糖苷酶具有浓度依赖性的抑制活性。与MCPa和MCPd相比，中等分子量的MCPb (Mw = 10.1 kDa)和MCPc (Mw = 5.62 kDa)表现出更高的抑制活性。图形抽象

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Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp. Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses. Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6. Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase. MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.