{"title":"荷斯坦奶牛和瑞士棕色奶牛MASP2、TG5和DQA1基因的遗传多态性","authors":"A. El Zeer, M. Fouda, R. Darwish, A. Ateya","doi":"10.12681/jhvms.29524","DOIUrl":null,"url":null,"abstract":"The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows\",\"authors\":\"A. El Zeer, M. Fouda, R. Darwish, A. Ateya\",\"doi\":\"10.12681/jhvms.29524\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.\",\"PeriodicalId\":17314,\"journal\":{\"name\":\"Journal of the Hellenic Veterinary Medical Society\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2023-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Hellenic Veterinary Medical Society\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.12681/jhvms.29524\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Hellenic Veterinary Medical Society","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.12681/jhvms.29524","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows
The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.
期刊介绍：
The Journal of the Hellenic Veterinary Medical Society (J Hellenic Vet Med Soc) is a quarterly peer-reviewed journal that publishes articles in all aspects of veterinary science and related disciplines. It is published by the Hellenic Veterinary Medical Society and is indexed in the Web of Science and in Scopus.
There are no publication fees in the journal. Authors considering submitting manuscripts for evaluation and publication are requested to read carefully the instructions for authors and fully comply with them.
Non-complying manuscripts may be returned to the corresponding author for formatting.
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