木霉菌株生产纤维素水解酶用于葡萄渣发酵作为固体底物再利用酒渣

Q2 Arts and Humanities Veritas Pub Date : 2021-11-19 DOI:10.35286/veritas.v22i1.290
Solange Grace Barrios Gutiérrez, Juan Diego Ernesto Guzmán Delgado, Claudia Valeria Navarrete Herrera, Miriam Rosario Flores Castro Linares, Hugo Guillermo Jiménez Pacheco
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引用次数: 0

摘要

目前,农业食品协会增加了对环境的浪费,并对环境产生了潜在影响,其中之一是葡萄酒行业,在短时间内产生了有机废物,这表明葡萄园中化肥、包装材料,他们的废物管理,如酿酒厂的葡萄生产和葡萄酒生产,对高污染的环境影响负有责任。在这方面,葡萄提取物和葡萄渣是多酚的丰富来源,因此在本研究中,我们评估了木霉菌株通过固态发酵(FES)获得纤维素水解酶的情况。使用了葡萄渣和葡萄茎残基,还向(FES)中添加了0.5%的羧甲基纤维素(CMC)和矿物溶液。结果表明,葡萄茎是一种比葡萄渣更好的基质。此外,(CMC)0.5%时的酶活性为0.1289U/mL,盐水溶液时的酶活力为0.1286U/mL。因此,从添加0.5%羧甲基纤维素的葡萄茎中,可以通过(FES)获得纤维素分解酶。这些结果为实验数据提供了回归系数的相关数据,从而表明纤维素酶生产对真菌生长及其酶分泌具有很高的潜力。
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PRODUCTION OF CELLULOLYTIC ENZYMES FROM TRICHODERMA SP. STRAINS FOR FERMENTATION OF GRAPE RESIDUES AS SOLID SUBSTRATE REUSING WINE RESIDUES
Currently, the agri-food society increases waste to the environment with potential environmental impacts and one of them is the wine industry, generating organic waste in short periods, showing that the use of fertilizers in the vineyard, packaging materials, and their waste management suchas grape production in wineries and wine production are responsible for environmental impacts that are highly polluting. In this aspect, grape extracts and grape pomace are rich sources of polyphenols, so in the present study, we evaluated the obtaining of cellulolytic enzymes, through fermentation in solid-state (FES) with strains of Trichoderma sp. Grape pomace and grape stalk residues were used, also Carboxymethylcellulose (CMC) at 0.5 % and mineral solution were added to the (FES). The results showed that grape stalk is a better substrate than grape pomace. In addition, the enzyme activity with (CMC) 0.5 % was 0.1289 U/mL and with saline solution was 0.1286 U/mL. Therefore, it is concluded that cellulolytic enzymes can be obtained by (FES) from grape stalk supplemented with CMC 0.5 %. These results provide relevant data on the regression coefficients to the experimental data resulting in the high potentiality of cellulase production about fungal growth and its enzymatic secretion.
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来源期刊
Veritas
Veritas Arts and Humanities-Religious Studies
CiteScore
0.40
自引率
0.00%
发文量
12
审稿时长
10 weeks
期刊介绍: VERITAS, Revista de Filosofía y Teología fue fundada en 1994 por el Pontificio Seminario Mayor San Rafael de Valparaíso (Chile). A partir del año 2017 es una publicación cuatrimestral (Abril, Agosto y Diciembre). El idioma habitual de la revista es el español, aunque queda abierta la posibilidad para publicar artículos en otros idiomas, tales como inglés, francés, italiano o portugués. VERITAS tiene como objetivo difundir entre los académicos y estudiantes del seminario, así como también de otras instituciones eclesiásticas y universitarias, nacionales y extranjeras, el resultado de la investigación en las áreas de la Filosofía y la Teología. Así, y desde su talante católico, pretende llevar a cabo una contribución de actualidad y rigor científico que promueva la reflexión y el debate abierto en la vida académica.
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