{"title":"Semaphorin 4C在结直肠癌癌症细胞发育中起关键作用","authors":"Hongyue Lin, Yuzhu Wu, Jinping Chen, Shurong Huang, Yang Zeng, Wei Zheng","doi":"10.1166/jbt.2023.3264","DOIUrl":null,"url":null,"abstract":"Objective: Purpose of this work was to discuss effects and mechanisms of Sema 4C in colon cancer development. Results: Sema4C were significantly upregulated in cancer tissues (P <0.001). Following transfection, the expression levels of Sema4C mRNA were significantly\n downregulated in the si-Sema4C groups compared with those in the si-negative control groups of both HT-29 and SW620 cells (both P <0.001). The apoptotic rate was significantly increased, while the invasive and wound healing rates were significantly suppressed in the si-Sema4C groups\n of HT-29 and SW620 cells (both P < 0.001). The results of the RT-qPCR and western blotting analyses revealed that PI3K, AKT, MMP-2 and MMP-9 mRNA and protein expression levels, respectively, were significantly downregulated, while caspase-3 mRNA and protein expression levels were\n significantly upregulated in the si-Sema4C groups of HT-29 and SW620 cells. Conclusion: The findings of the present study demonstrated that the knockdown of Sema4C expression suppressed CRC cell biological activities by regulating the PI3K/AKT signaling pathway. Therefore, Sema4C may\n act as an oncogene in CRC.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Semaphorin 4C Plays a Key Role in Colorectal Cancer Cells Development\",\"authors\":\"Hongyue Lin, Yuzhu Wu, Jinping Chen, Shurong Huang, Yang Zeng, Wei Zheng\",\"doi\":\"10.1166/jbt.2023.3264\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: Purpose of this work was to discuss effects and mechanisms of Sema 4C in colon cancer development. Results: Sema4C were significantly upregulated in cancer tissues (P <0.001). Following transfection, the expression levels of Sema4C mRNA were significantly\\n downregulated in the si-Sema4C groups compared with those in the si-negative control groups of both HT-29 and SW620 cells (both P <0.001). The apoptotic rate was significantly increased, while the invasive and wound healing rates were significantly suppressed in the si-Sema4C groups\\n of HT-29 and SW620 cells (both P < 0.001). The results of the RT-qPCR and western blotting analyses revealed that PI3K, AKT, MMP-2 and MMP-9 mRNA and protein expression levels, respectively, were significantly downregulated, while caspase-3 mRNA and protein expression levels were\\n significantly upregulated in the si-Sema4C groups of HT-29 and SW620 cells. Conclusion: The findings of the present study demonstrated that the knockdown of Sema4C expression suppressed CRC cell biological activities by regulating the PI3K/AKT signaling pathway. Therefore, Sema4C may\\n act as an oncogene in CRC.\",\"PeriodicalId\":15300,\"journal\":{\"name\":\"Journal of Biomaterials and Tissue Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomaterials and Tissue Engineering\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1166/jbt.2023.3264\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials and Tissue Engineering","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1166/jbt.2023.3264","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Semaphorin 4C Plays a Key Role in Colorectal Cancer Cells Development
Objective: Purpose of this work was to discuss effects and mechanisms of Sema 4C in colon cancer development. Results: Sema4C were significantly upregulated in cancer tissues (P <0.001). Following transfection, the expression levels of Sema4C mRNA were significantly
downregulated in the si-Sema4C groups compared with those in the si-negative control groups of both HT-29 and SW620 cells (both P <0.001). The apoptotic rate was significantly increased, while the invasive and wound healing rates were significantly suppressed in the si-Sema4C groups
of HT-29 and SW620 cells (both P < 0.001). The results of the RT-qPCR and western blotting analyses revealed that PI3K, AKT, MMP-2 and MMP-9 mRNA and protein expression levels, respectively, were significantly downregulated, while caspase-3 mRNA and protein expression levels were
significantly upregulated in the si-Sema4C groups of HT-29 and SW620 cells. Conclusion: The findings of the present study demonstrated that the knockdown of Sema4C expression suppressed CRC cell biological activities by regulating the PI3K/AKT signaling pathway. Therefore, Sema4C may
act as an oncogene in CRC.