多菌株益生菌提取物通过抑制巨噬细胞迁移抑制因子活性发挥抗炎作用的潜力

IF 1.3 Q4 FOOD SCIENCE & TECHNOLOGY Functional Foods in Health and Disease Pub Date : 2023-01-04 DOI:10.31989/ffhd.v13i1.1033
Denny Nyotohadi, T. Go
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引用次数: 3

摘要

背景:据报道,益生菌有改善健康状况和降低炎症相关疾病风险的作用。然而,它们如何影响炎症还没有得到很好的研究。炎症发生在慢性疾病的发展过程中,可能会损害细胞、组织和器官的正常功能。巨噬细胞迁移抑制因子(MIF)被认为是一种在炎症过程中发挥关键作用的细胞因子。研究人员已经将抑制其活性作为减轻炎症的一种方法。本研究旨在评估由干酪乳杆菌EMRO 002、干酪乳杆菌EMRO 213、植物乳杆菌EMRO 009、干酪乳菌EMRO 213组成的多菌株益生菌提取物的潜在抑制作用。fermentum EMRO 211、L.rhamnosus EMRO 014、L.bulgaricus EMRO 212和Rhodopseudomonas palustris EMRO 201对MIF互变异构酶活性、可逆性和抑制机制的影响。因此,本研究的范围是评估多菌株益生菌提取物对MIF活性的功能特性。方法:对多菌株益生菌进行离心分离,从沉淀中分离上清液。将上清液(即提取物)与MIF混合,并分析其对MIF互变异构酶活性的抑制作用。通过预培养和稀释试验评估抑制的可逆性,并通过动力学评估确定抑制机制。结果:多菌株益生菌提取物抑制MIF互变异构酶活性,IC50为7.80±1.96mg/L。预培养和稀释试验表明,抑制作用是可逆的,动力学评价预测提取物组分可能与MIF的酶活性位点和其他位点结合用于治疗与炎症相关的慢性疾病的抗炎剂。关键词:多菌种益生菌,抗炎,巨噬细胞迁移抑制因子,可逆抑制,动力学评价。
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Potential of multi-strain probiotics extract as an anti-inflammatory agent through inhibition of macrophage migration inhibitory factor activity
Background: Probiotics are reported to have a role for improving health conditions and reduce the risk of diseases associated with inflammation. However, how they affect inflammation has not been well studied. The inflammation occurs during the progression of chronic diseases could damage the normal function of cells, tissues, and organs. Macrophage migration inhibitory factor (MIF) is recognized as a cytokine playing a key role in the inflammation process. Inhibition of its activity has been used by researchers as an approach for alleviating the inflammation. This study aims to evaluate the potential inhibitory effect of the extract of multi-strain probiotics consisting of Lactobacillus casei EMRO 002, L. casei EMRO 213, L. plantarum EMRO 009, L. fermentum EMRO 211, L. rhamnosus EMRO 014, L. bulgaricus EMRO 212,and Rhodopseudomonas palustris EMRO 201 on MIF tautomerase activity, the reversibility, and the mechanism of inhibition. Hence, the scope of this study is the evaluation of the functional characteristics of multi-strain probiotics extract on MIF activity.Methods:The multi-strain probiotics were centrifuged and the supernatant was separated from the pellet. The supernatant, i.e. the extract, was mixed with MIF and analyzed for its inhibition effect on MIF tautomerase activity. The reversibility of inhibition was evaluated by preincubation and dilution assays, and the mechanism of inhibition was determined by kinetic evaluation.Results:The multi-strain probiotics extract inhibited MIF tautomerase activity with an IC50 of 7.80 ± 1.96 mg/L. The preincubation and dilution assays showed that the inhibition is reversible, and the kinetic evaluation predicted that the extract components might bind to the enzyme active site and other site(s) of MIF.Conclusion:The findings show that the extract was able to inhibit MIF tautomerase activity reversibly and its components might bind to the enzyme active site and other site(s) of MIF suggest that the multi-strain probiotics extract has potential to be used as an anti-inflammatory agent for the treatment of chronic diseases associated with inflammation.Keywords: Multi-strain probiotics, anti-inflammatory, macrophage migration inhibitory factor, reversible inhibition, kinetic evaluation. 
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来源期刊
Functional Foods in Health and Disease
Functional Foods in Health and Disease FOOD SCIENCE & TECHNOLOGY-
CiteScore
2.20
自引率
20.00%
发文量
47
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