用液态水浮液(LBF)和对抗氧化剂活性的测定，从螺旋体中提炼出雄激素

Jurnal Riset Kimia Pub Date : 2021-09-09 DOI:10.25077/jrk.v12i2.412

Cori Yolanda Elise, Syafrizayanti Syafrizayanti, Marniati Salim

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引用次数: 0

摘要

螺旋藻藻蓝蛋白(藻蓝蛋白)是一种色素复合物蛋白，属于捕光藻胆蛋白家族。该色素作为天然蓝色染料具有很高的经济价值，同时也是抗氧化剂和抗癌物质的来源。纯天然藻蓝蛋白的生产仍然有很高的需求。因此，本研究旨在采用改性液体双相浮选(LBF)体系获得高纯度的藻蓝蛋白，并对DPPH(1,1-二苯基-2-吡啶肼)自由基进行清除活性测试。本研究获得了高纯度藻蓝蛋白，纯化倍数为3.041±0.04，回收率约为70.881%。纯化的藻蓝蛋白具有清除活性，IC50为338.585 mg/mL。因此，LBF系统产生了高纯度的藻蓝蛋白色素。

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Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of 338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.

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Jurnal Riset Kimia

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12 weeks