表皮生长因子负载微球/水凝胶复合材料用于即时止血和肝脏再生

Q1 Engineering Smart Materials in Medicine Pub Date : 2023-01-01 DOI:10.1016/j.smaim.2022.09.006
Rui Ding , Xinbo Wei , Youlan Liu, Yuqing Wang, Zheng Xing, Li Wang, Haifeng Liu, Yubo Fan
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引用次数: 4

摘要

对于无法缝合的不可压缩性肝伤口,特别是大面积肝伤口的快速止血和有效愈合,仍然是临床面临的巨大挑战。本研究制备了负载表皮生长因子(EGF)的壳聚糖微球(CM),并将其加入光交联明胶甲基丙烯酰(GelMA)水凝胶中。结果表明,负载egf的CM/GelMA前体溶液可以转化为水凝胶,并在无外部应力的情况下在撕裂处止血。随后,EGF的持续释放加速伤口愈合,促进肝脏再生。体外实验表明,微球/水凝胶复合材料能促进L02细胞的增殖和迁移。此外,组织学和免疫组织学分析表明,EGF-CM/GelMA复合物可以减轻小鼠肝脏炎症,促进肝脏重塑。总之,这种多功能微球/水凝胶复合材料将激发不可压缩止血和连续伤口闭合的临床应用的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Epidermal growth factor-loaded microspheres/hydrogel composite for instant hemostasis and liver regeneration

Rapid hemostasis and effective healing for the non-compressible liver wounds which are not able to be sewn, especially for those large-area wounds, remain great clinical challenges. In this study, we fabricated epidermal growth factor (EGF)-loaded chitosan microspheres (CM) and then incorporated them into a photo-crosslinking gelatin methacryloyl (GelMA) hydrogel. The results showed that the EGF-loaded CM/GelMA precursor solution could transform into a hydrogel and cease bleeding at laceration sites without external stress. Subsequently, the sustained release of EGF accelerated wound closure and promoted liver regeneration. The in vitro experiments demonstrated that the microsphere/hydrogel composite could promote the proliferation and migration of L02 ​cells. Moreover, the histological and immunohistological analyses indicated that EGF-CM/GelMA composite could alleviate inflammation in the mouse liver and promote liver remodeling. Overall, this multi-functional microsphere/hydrogel composite will inspire the development of clinical applications for noncompressible hemostasis and successive wound closure.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
期刊最新文献
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