从Kendit Water提取试验型马氏海绵作为大肠杆菌的抗菌潜力

IF 0.5 4区 地球科学 Q3 Earth and Planetary Sciences Journal of Marine Research Pub Date : 2023-02-01 DOI:10.14710/jmr.v12i1.36278
D. Maisaroh, Yahya Abdillah Al Hanif, M. Munir, Nor Sa’adah
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引用次数: 0

摘要

用于了解所含化合物的生物活性潜力,并了解Ptilocaulis marquezii赞助商对大肠杆菌生长的提取物测试。实验研究是通过按摩和用旋转真空蒸发器在40°C下蒸发直到形成提取物来提取马齿苋海绵的提取物试验。使用圆盘扩散或Kirby-Bauer测试进行测试。[UNK]用于测定化合物活性物质的植物化学测试。所获得的结果是在具有电流能力的40%的浓度下提取的。植物化学测试含有酒精和三萜类化合物。在当前类别中,以40%的浓度和9.5 mm的缓冲区提取Ptilocaulis marquezii海绵,在另一个浓度为10%、20%、60%和80%的弱类别中提取。生物碱化合物通过破坏肽聚糖序列来抑制大肠杆菌的生长。在一种三萜化合物中,它会破坏大肠杆菌的质膜。了解所含化合物的潜在生物活性,并了解Eschercia coli细菌的Ptilocaulis marquezii海绵提取物的测试。这项研究是以提取物测试的形式进行的,即通过在40°C的温度下用旋转真空蒸发器浸渍和蒸发制备马齿苋海绵提取物,直到形成提取物。该试验使用圆盘扩散法或Kirby-Bauer试验进行。植物化学测试,以确定活性化合物的含量。所获得的结果是具有中等能力的浓度为40%的提取物。植物化学测试含有生物碱和三萜类化合物。在中等类别中,浓度为40%、抑制区为9.5 mm的马氏Ptilocaulis海绵提取物,在弱类别中,其他浓度为10%、20%、60%和80%。生物碱通过破坏肽聚糖结构来抑制大肠杆菌的生长。三萜类化合物会破坏大肠杆菌的质膜。
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Uji Ekstrak Spons Laut Jenis Ptilocaulis marquezii dari Perairan Kendit sebagai Potensi Antibakteri Escherichia coli
Guna mengetahui potensi bioaktivitas senyawa yang terkandung serta mengetahui uji ekstrak spons Ptilocaulis marquezii terhadap pertumbuhan bakteri Eschercia coli. Penelitian dilakukan secara eksperimen berupa uji ekstrak berupa pembuatan ekstrak spons Ptilocaulis marquezii dengan maserasi serta evaporasi dengan rotary vacuum evaporator pada suhu 40°C hingga ekstrak terbentuk. Dilakukan pengujian dengan metode Difusi cakram atau Kirby-Bauer test.  Pengujian fitokimia guna mengetahui kandungan senyawa aktif. Hasil yang diperoleh didapatkan ekstrak dengan konsentrasi 40% yang memiliki kemampuan sedang. Pada uji fitokimia mengandung alkoloid dan triptenoid. Ekstrak spons Ptilocaulis marquezii pada konsentrasi 40% dengan zona hambat sebesar 9.5 mm dalam kategori sedang dan pada konsentrasi lainnya yaitu 10%, 20%, 60% dan 80% berkategori lemah. Senyawa alkaloid menghambat pertumbuhan Escherichia Coli dengan merusak susunan peptidoglycan. Pada senyawa triterpenoid merusak membran plasma pada bakteri Escherichia coli.  Knowing the potential bioactivity of the compounds contained and knowing the test of the extract of the Ptilocaulis marquezii sponge of Eschercia coli bacteria. The research was carried out experimentally in the form of extract testing in the form of making Ptilocaulis marquezii sponge extract by maceration and evaporation with a rotary vacuum evaporator at a temperature of 40°C until the extract was formed. The test was carried out using the disc diffusion method or the Kirby-Bauer test. Phytochemical testing to determine the content of active compounds. The results obtained were extracts with a concentration of 40% which had moderate abilities. The phytochemical test contains alkaloids and triptenoids. Ptilocaulis marquezii sponge extract at a concentration of 40% with an inhibition zone of 9.5 mm in the medium category and at other concentrations of 10%, 20%, 60% and 80% in the weak category. Alkaloids inhibit the growth of Escherichia Coli by destroying the peptidoglycan structure. The triterpenoid compounds damage the plasma membrane of Escherichia coli bacteria.
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Journal of Marine Research
Journal of Marine Research 地学-海洋学
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3 months
期刊介绍: The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.
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