Wanling Wu, Zhiqi Li, Guangqing Liu, Ling Zhou, Wen Wang
{"title":"厌氧CO2发酵对C2‐C8羧酸生物合成的调控","authors":"Wanling Wu, Zhiqi Li, Guangqing Liu, Ling Zhou, Wen Wang","doi":"10.1002/elsc.202200069","DOIUrl":null,"url":null,"abstract":"<p>Bioconversion of CO<sub>2</sub> into liquid fuels or chemicals, preferred medium chain carboxylic acids (caproic and caprylic acid), is an attractive CO<sub>2</sub> utilization technology. The present study aims to investigate the effects of different ratios of H<sub>2</sub>/CO<sub>2</sub> on regulating the distribution of C2-C8 carboxylic acid products, while the headspace pressure of 1.5 bar was set to amplify the effect of different ratios. The H<sub>2</sub>/CO<sub>2</sub> ratio of 4:1 was more suitable for preparing acetic acid, where the highest acetic acid yield was 17.5 g/L. And the H<sub>2</sub>/CO<sub>2</sub> ratio of 2:1 showed excellent chain elongation ability with the highest n-caprylic yield of 2.4 g/L. Additionally, the actual H<sub>2</sub>/CO<sub>2</sub> ratios of 4:1 reactors were higher than that in 2:1 may be course chain elongation often accompanied by H<sub>2</sub> production. The 16S rRNA genes analysis shows that the genus <i>Terrisporobacter</i> and <i>Coriobacteriales</i> may be related to acetic acid production enriched in H<sub>2</sub>/CO<sub>2</sub> ratio 4:1 reactors, and the genus <i>Clostridium</i> and <i>Paenibacillaceae</i> may associate with the chain elongation pathway were enriched in H<sub>2</sub>/CO<sub>2</sub> ratio 2:1 reactors.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 5","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202200069","citationCount":"0","resultStr":"{\"title\":\"Regulation on C2-C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation\",\"authors\":\"Wanling Wu, Zhiqi Li, Guangqing Liu, Ling Zhou, Wen Wang\",\"doi\":\"10.1002/elsc.202200069\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Bioconversion of CO<sub>2</sub> into liquid fuels or chemicals, preferred medium chain carboxylic acids (caproic and caprylic acid), is an attractive CO<sub>2</sub> utilization technology. The present study aims to investigate the effects of different ratios of H<sub>2</sub>/CO<sub>2</sub> on regulating the distribution of C2-C8 carboxylic acid products, while the headspace pressure of 1.5 bar was set to amplify the effect of different ratios. The H<sub>2</sub>/CO<sub>2</sub> ratio of 4:1 was more suitable for preparing acetic acid, where the highest acetic acid yield was 17.5 g/L. And the H<sub>2</sub>/CO<sub>2</sub> ratio of 2:1 showed excellent chain elongation ability with the highest n-caprylic yield of 2.4 g/L. Additionally, the actual H<sub>2</sub>/CO<sub>2</sub> ratios of 4:1 reactors were higher than that in 2:1 may be course chain elongation often accompanied by H<sub>2</sub> production. The 16S rRNA genes analysis shows that the genus <i>Terrisporobacter</i> and <i>Coriobacteriales</i> may be related to acetic acid production enriched in H<sub>2</sub>/CO<sub>2</sub> ratio 4:1 reactors, and the genus <i>Clostridium</i> and <i>Paenibacillaceae</i> may associate with the chain elongation pathway were enriched in H<sub>2</sub>/CO<sub>2</sub> ratio 2:1 reactors.</p>\",\"PeriodicalId\":11678,\"journal\":{\"name\":\"Engineering in Life Sciences\",\"volume\":\"24 5\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2022-12-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202200069\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Engineering in Life Sciences\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/elsc.202200069\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Engineering in Life Sciences","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/elsc.202200069","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Regulation on C2-C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation
Bioconversion of CO2 into liquid fuels or chemicals, preferred medium chain carboxylic acids (caproic and caprylic acid), is an attractive CO2 utilization technology. The present study aims to investigate the effects of different ratios of H2/CO2 on regulating the distribution of C2-C8 carboxylic acid products, while the headspace pressure of 1.5 bar was set to amplify the effect of different ratios. The H2/CO2 ratio of 4:1 was more suitable for preparing acetic acid, where the highest acetic acid yield was 17.5 g/L. And the H2/CO2 ratio of 2:1 showed excellent chain elongation ability with the highest n-caprylic yield of 2.4 g/L. Additionally, the actual H2/CO2 ratios of 4:1 reactors were higher than that in 2:1 may be course chain elongation often accompanied by H2 production. The 16S rRNA genes analysis shows that the genus Terrisporobacter and Coriobacteriales may be related to acetic acid production enriched in H2/CO2 ratio 4:1 reactors, and the genus Clostridium and Paenibacillaceae may associate with the chain elongation pathway were enriched in H2/CO2 ratio 2:1 reactors.
期刊介绍：
Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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