Guo Qiulei , Liu Qingguo , Sun Dongmei , Nie Binbin
{"title":"旋转补泻手法对自发性高血压大鼠降压作用的中心机制","authors":"Guo Qiulei , Liu Qingguo , Sun Dongmei , Nie Binbin","doi":"10.1016/S0254-6272(18)30629-0","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation (TRRM) might be the central mechanism underlying the action.</p></div><div><h3>METHODS</h3><p>In the study, <span><sup>18</sup></span>F-2-fluoro-deoxy-D-glucose positron emission tomography (<span><sup>18</sup></span>F-FDG-PET) was employed. Fifity-six spontaneous hypertensive rats (SHRs) were randomly divided into a model group, a single-needle acupuncture (SNA) group, a twirling reinforcing group (SNA + TRF) and a twirling reducing (SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong (LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure (BP) of all rats was measured repeatedly and <span><sup>18</sup></span>F-FDG-PET scans were conducted on the 14th day. PET images were processed with Statistical Parametric Mapping 8.0.</p></div><div><h3>RESULTS</h3><p>After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups (all <em>P</em> < 0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups (both <em>P</em> < 0.01), with the SNA + TRD group exhibited the best antihypertensive effect (<em>P</em> < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.</p></div><div><h3>CONCLUTION</h3><p>TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 391-398"},"PeriodicalIF":2.0000,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30629-0","citationCount":"4","resultStr":"{\"title\":\"Twirling reinforcing-reducing manipulation — central mechanism underlying antihypertensive effect on spontaneous hypertension in rats\",\"authors\":\"Guo Qiulei , Liu Qingguo , Sun Dongmei , Nie Binbin\",\"doi\":\"10.1016/S0254-6272(18)30629-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation (TRRM) might be the central mechanism underlying the action.</p></div><div><h3>METHODS</h3><p>In the study, <span><sup>18</sup></span>F-2-fluoro-deoxy-D-glucose positron emission tomography (<span><sup>18</sup></span>F-FDG-PET) was employed. Fifity-six spontaneous hypertensive rats (SHRs) were randomly divided into a model group, a single-needle acupuncture (SNA) group, a twirling reinforcing group (SNA + TRF) and a twirling reducing (SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong (LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure (BP) of all rats was measured repeatedly and <span><sup>18</sup></span>F-FDG-PET scans were conducted on the 14th day. PET images were processed with Statistical Parametric Mapping 8.0.</p></div><div><h3>RESULTS</h3><p>After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups (all <em>P</em> < 0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups (both <em>P</em> < 0.01), with the SNA + TRD group exhibited the best antihypertensive effect (<em>P</em> < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.</p></div><div><h3>CONCLUTION</h3><p>TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.</p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":\"38 3\",\"pages\":\"Pages 391-398\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30629-0\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627218306290\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627218306290","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Twirling reinforcing-reducing manipulation — central mechanism underlying antihypertensive effect on spontaneous hypertension in rats
OBJECTIVE
To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation (TRRM) might be the central mechanism underlying the action.
METHODS
In the study, 18F-2-fluoro-deoxy-D-glucose positron emission tomography (18F-FDG-PET) was employed. Fifity-six spontaneous hypertensive rats (SHRs) were randomly divided into a model group, a single-needle acupuncture (SNA) group, a twirling reinforcing group (SNA + TRF) and a twirling reducing (SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong (LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure (BP) of all rats was measured repeatedly and 18F-FDG-PET scans were conducted on the 14th day. PET images were processed with Statistical Parametric Mapping 8.0.
RESULTS
After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups (all P < 0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups (both P < 0.01), with the SNA + TRD group exhibited the best antihypertensive effect (P < 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.
CONCLUTION
TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.
期刊介绍:
Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.