美国弗吉尼亚州阿巴拉契亚自然隧道州立公园淡水浮游原生生物的元条形码普查

A. B. Cahoon, Ashley G. Huffman, Megan M. Krager, Roseanna M. Crowell
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引用次数: 11

摘要

我们研究的目的是使用metabarcoding调查阿巴拉契亚地区山脊和山谷自然地理省内陆自然保护区内的淡水浮游原生生物。微生物真核生物是小型淡水生态系统中重要的初级生产者和捕食者,但由于难以识别,它们经常被忽视。这在一定程度上通过高通量DNA测序的成本降低和条形码数据库的增长得到了弥补,使得在复杂的生态系统中通过代谢条形码调查来识别和分析微生物变得越来越可行。从弗吉尼亚州斯科特县自然隧道州立公园的五个地点采集了水样,代表了该地区发现的三种常见水体。样本最初是在2016年4月的一次生物闪电战活动中收集的,之后的7周和14周。对23S和18S基因的Metabarcode分析确定了3663个OTU,代表213个科级和332个属级分类群。这项研究提供了一个以温带生物多样性“热点”著称的地区的初步条形码普查。原生生物的总体多样性与其他温带系统相当高,但并不异常高;然而,微藻的多样性高于其他温带地区的报道。这三种类型的水体都有自己独特的原生生物群落,尽管距离很近。
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A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA
The purpose of our study was to survey the freshwater planktonic protists within an inland natural preserve in the Ridge and Valley physiographic province of the Appalachian Region using metabarcoding. Microbial eukaryotes are essential primary producers and predators in small freshwater ecosystems, yet they are often overlooked due to the difficulty of identification. This has been remedied, in part, by the cost reduction of high throughput DNA sequencing and the growth of barcode databases, making the identification and analysis of microorganisms by way of metabarcoding surveys in complex ecosystems increasingly feasible. Water samples were collected from five sites at the Natural Tunnel State Park in Scott County, VA (USA), representing three common bodies of water found in this region. Samples were initially collected during a Bioblitz event in April 2016 and then seven and fourteen weeks afterwards. Metabarcode analysis of the 23S and 18S genes identified 3663 OTUs representing 213 family level and 332 genus level taxa. This study provides an initial barcode census within a region that has a reputation as a temperate biodiversity “hotspot”. The overall protist diversity was comparably high to other temperate systems, but not unusually high; the microalgal diversity, however, was higher than that reported for other temperate regions. The three types of water bodies had their own distinctive protist biomes despite close proximity.
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来源期刊
Metabarcoding and Metagenomics
Metabarcoding and Metagenomics Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
5.40
自引率
0.00%
发文量
25
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