红高良姜提取物对迟发芽孢杆菌生长的抑菌作用

Desy Amalia Hidayati, A. Prajitno, Titik Dwi Sulistyawati, Giri Pratama, Tania Nilakandhi
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引用次数: 1

摘要

本研究旨在分析红高良姜提取物(Alpinia purpurata)对E.tarda生长的抗菌活性。本研究的目的是确定红高良姜提取物(A.purpurata)的抗菌化合物和对E.tarda细菌生长的抗菌效果。采用MIC法和纸片法进行抗菌活性试验。MIC测试使用1ppm、10ppm、100ppm、500ppm和1000ppm的剂量,孵育24小时。椎间盘试验使用了100ppm、200ppm、300ppm、400ppm、500ppm的剂量以及5ppm氯霉素的阳性对照和未经治疗的阴性对照。红高良姜提取物(A.purpurata)含有黄酮类化合物、生物碱、单宁、三萜和皂苷。圆盘试验的结果表明,在500ppm的剂量下,最高抑制直径为7.55mm,并且是抑菌的,因为它在培养48小时后降低了其抑制区。培养24小时后,在500ppm的剂量下,最高的抑制有效性为56.56%。
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Antibacterial Activity of Red Galangal (A. purpurata) Extract on the Growth of E. tarda Bacteria
This study aimed to analyze the antibacterial activity of red galangal extract (Alpinia purpurata) against the growth of E. tarda. This aims of this study was to determine the antibacterial compounds and the antibacterial effectiveness of red galangal extract (A. purpurata) against the growth of E. tarda bacteria. Antibacterial activity test was carried out by MIC test and disc test. The MIC test used doses of 1 ppm, 10 ppm, 100 ppm, 500 ppm, and 1000 ppm with incubation for 24 hours. The disc test used doses of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm and a positive control of 5 ppm chloramphenicol and a negative control without treatment. Photochemical test of red galangal extract (A. purpurata) contains flavonoid compounds, alkaloids, tannins, triterpenoids, and saponins. The results of the disc test showed that the highest inhibitory diameter was 7.55 mm at a dose of 500 ppm and was bacteriostatic because it decreased its inhibitory zone after 48 hours of incubation. The highest inhibitory effectiveness was 56.56% at a dose of 500 ppm after 24 hours of incubation.
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发文量
28
审稿时长
6 weeks
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