大豆秸秆水热预处理液对纤维素酶活性的抑制作用

IF 2.5 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Frontiers in chemical engineering Pub Date : 2022-11-21 DOI:10.3389/fceng.2022.1004240
Daehwan Kim, Anqi Ji, Armoni L. Jackson, Bailee Brown, Youngmi Kim, S. Kim, C. Laufer, Drew Ferrier, C. Yoo
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引用次数: 0

摘要

一锅生物质转化工艺是一种很有前途的策略,可以最大限度地减少潜在的产品损失并降低加工成本。然而,由于木质素等生物质成分以及生物质加工过程中产生的抑制剂(如呋喃、酚类)的抑制作用,该策略具有技术局限性。研究了氢氧化钠(NaOH)或过氧化氢(H2O2)水热预处理大豆秸秆形成的液体水解产物对纤维素酶活性的抑制作用。用氢氧化钠(1%v/v)或过氧化氢(1%v/v)在121°C下对大豆秸秆(10%w/v)进行60分钟的水热预处理,以评估大豆预处理释放的水溶性抑制剂对纤维素酶活性的影响。在预处理产生的缓冲液或液体水解产物存在下,用45IU/g葡聚糖(相当于25mg酶蛋白/g葡聚糖)对预处理固体中的纤维素部分(1%w/v葡聚糖)进行酶水解72小时。NaOH和H2O2预处理固体的水解导致缓冲液中葡萄糖产量分别为57%和39%。在液体水解产物存在的情况下,NaOH和H2O2预处理的生物质分别显示出20%和30%的葡萄糖产率,表明液体水解产物中的抑制剂对酶的抑制作用。在测试的水解产物中的酶活性中,与H2O2液体相比,NaOH水解产物对酶活性(主要是β-葡萄糖苷酶)表现出更高的抑制作用,其中酶失活与预处理的大豆秸秆产生真空过滤抑制剂的方式具有一级相关性。
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Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw
The one-pot biomass conversion process is a promising strategy to minimize potential product loss and reduce processing costs. However, this strategy has technical limitations due to the inhibitory effects of biomass components like lignin as well as the generated inhibitors (e.g., furans, phenols) during biomass processing. In this study, the inhibitory effects of liquid hydrolysates formed by hydrothermal pretreatment of soybean straw with either sodium hydroxide (NaOH) or hydrogen peroxide (H2O2) on cellulolytic enzyme activity were investigated. Hydrothermal pretreatment of soybean straw (10% w/v) was carried out with either sodium hydroxide (1% v/v) or hydrogen peroxide (1% v/v) at 121°C for 60 min to evaluate the effect of water-soluble inhibitors released from soybean pretreatment on cellulolytic enzyme activity. The fraction of cellulose in pretreated solids (1% w/v glucan) was enzymatically hydrolyzed for 72 h with 45 IU/g glucan (corresponding to 25 mg enzyme protein/g glucan) in the presence of either buffer or liquid hydrolysate generated from the pretreatments. Hydrolysis of NaOH and H2O2 pretreated solids resulted in 57% and 39% of glucose yields in buffer, respectively. In the presence of the liquid hydrolysates, NaOH and H2O2 pretreated biomass showed 20% and 30% glucose yield, respectively, indicating the enzyme suppression by inhibitors in the liquid hydrolysates. Of the enzyme activities in hydrolysates tested, NaOH hydrolysate showed a higher inhibitory effect on enzyme activities (mainly β-glucosidase) compared to H2O2 liquid, where enzyme deactivation has a first-order correlation and the manner in which the vacuum-filtered inhibitors were generated from pretreated soybean straw.
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CiteScore
3.50
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0.00%
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0
审稿时长
13 weeks
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