E. D. Quintela, Thiago Livio Pessoa Oliveira de Souza, Josias Correa Faria, Francisco José Lima Aragão, Jose Francisco Arruda e Silva, M. J. Del Peloso, S. Arthurs
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In 2 field trials, B. tabaci established on all varieties, but showed preference for the conventional cultivars ‘Pérola’ and ‘BRS Pontal’ when compared with elite lines (averaging > 400 nymphs per leaflet, and > 97% infection rate in 1 study). However, whereas elite lines were resistant to Bean golden mosaic virus, all became infected with Cowpea mild mottle virus. Highest infection rates (19 to 99% infected plants) occurred in genetically modified elite lines derived from BRS Pontal versus Pérola (12 to 16%). When comparing seed yield, most elite lines outperformed their conventional recurrent parent. On average, elite lines achieved relative yield increases between 199 and 1,045%, and the varieties CNFCT 16205 and CNFCT 16210 were the most productive in our field trials. Our results showed that the use of common bean cultivars resistant to golden mosaic is an important tool within integrated management strategies for whiteflies and viruses. However, further studies are needed to elucidate the threat of Cowpea mild mottle virus and the nature of whitefly interactions between these different viruses. Resumo No Brasil, o Cowpea mild mottle virus transmitido pela Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), é uma ameaça emergente ao feijão comum (Phaseolus vulgaris L.; Fabaceae), incluindo as linhas elites de feijão geneticamente modificadas desenvolvidas para resistência ao Bean golden mosaic virus. Para investigar essa interação, quantificamos a suscetibilidade de dez linhas geneticamente modificadas resistentes ao Bean golden mosaic virus à infecção pelo Cowpea mild mottle virus em duas regiões do Brasil com incidência natural de B. tabaci virulíferas. Em dois ensaios de campo, B. tabaci estabeleceu em todas as variedades, mas mostrou preferência pelas cultivares convencionais ‘Pérola’ e ‘BRS Pontal’ quando comparadas com as linhas elite geneticamente modificadas (média > 400 ninfas por folíolo e > 97% taxa de infecção por virus em um dos estudos). No entanto, embora as linhas elite foram resistentes ao Bean golden mosaic virus, todas foram infectadas pelo Cowpea mild mottle virus. As maiores taxas de infecção (19 a 99% de plantas infectadas) ocorreram nas linhas elite geneticamente modificadas derivadas da BRS Pontal versus Pérola (12 a 16%). Ao comparar a produtividade, a maioria das linhas elite superou seu pai convencional recorrente. Os aumentos relativos de rendimento foi de 199 a 1.045% sendo as variedades CNFCT 16205 e CNFCT 16210 as mais produtivas. Nossos resultados mostraram que o uso de cultivares de feijão resistentes ao mosaico dourado é uma ferramenta importante dentro das estratégias integradas de manejo para moscas-brancas e vírus. No entanto, mais estudos são necessários para elucidar à ameaça do Cowpea mild mottle virus e a natureza das interações da mosca-branca com estes diferentes vírus.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of Bemisia tabaci Infestation, Virus Infection, and Yield in Conventional and Transgenic Bean Golden Mosaic Virus-Resistant Common Bean Elite Lines\",\"authors\":\"E. D. Quintela, Thiago Livio Pessoa Oliveira de Souza, Josias Correa Faria, Francisco José Lima Aragão, Jose Francisco Arruda e Silva, M. J. Del Peloso, S. 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引用次数: 0
摘要
摘要在巴西,以烟粉虱(半翅目:烟粉虱科)为媒介的豇豆轻度斑纹病毒是一种新兴的威胁菜豆(Phaseolus vulgaris L.;蚕豆科),包括为抵抗蚕豆金色花叶病毒而开发的转基因优良豆系。为了研究这种相互作用,我们定量分析了10个大豆金花叶病毒抗性品系在巴西2个有毒性烟粉虱自然发病的地区对豇豆轻度斑疹病毒感染的敏感性。在2个田间试验中,烟粉虱在所有品种上都有繁殖,但与优良品系相比,烟粉虱对常规品种“pembrorola”和“BRS Pontal”表现出更强的偏好(平均每小叶400个螨,1个研究的侵染率为97%)。优良品系对豆金花叶病毒具有抗性,但均感染了豇豆轻度斑驳病毒。最高的侵染率(19 - 99%)发生在由BRS Pontal和psamola衍生的转基因精品系(12 - 16%)。在比较种子产量时,大多数优良系的表现优于常规的循环亲本。平均而言,优质品系的相对产量提高在199 ~ 1045%之间,其中CNFCT 16205和CNFCT 16210在田间试验中产量最高。本研究结果表明,利用抗金花叶病的普通豆品种是白蝇和病毒综合管理策略的重要工具。然而,需要进一步的研究来阐明豇豆轻度斑驳病毒的威胁以及这些不同病毒之间白蝇相互作用的性质。巴西恢复,o豇豆轻度斑疹病毒(半翅目:烟粉虱科),巴西豇豆轻度斑疹病毒(半翅目:烟粉虱科);蚕豆科),包括linhas精英de feij o遗传修饰的desenvolvidas para resistência ao蚕豆金花叶病毒。对大豆金花叶病毒感染的基因修饰及其抗性进行了定量研究regiões do Brasil com incidência天然tabaci virulíferas。烟草芽孢杆菌是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌,是一种烟草芽孢杆菌。无独有偶,有孔虫对蚕豆金花叶病毒具有优良的抗性,今天有孔虫侵染蚕豆轻度斑驳病毒。作为主要的taxas de infectadada o (19 / 99% de plantas infectadas), ocreram在遗传修饰上有优势,如BRS Pontal和psamicada(12 / 16%)。与生产水平相比,大多数中国企业都拥有精英企业,而不是传统企业。以CNFCT 16205和CNFCT 16210为主要产品,其相对于1999年的产量为1.045%。nossso的研究结果表明,在不同的栽培品种中,有不同种类的植物具有不同的抗性;在不同的栽培品种中,有不同种类的植物具有不同的抗性;在不同的栽培品种中,有不同种类的植物具有不同的抗性。没有进入,主要的estudos s o necessários para elucidar a do豇豆轻度斑驳病毒ea nature as interações da mosca-科com es差异vírus。
Comparison of Bemisia tabaci Infestation, Virus Infection, and Yield in Conventional and Transgenic Bean Golden Mosaic Virus-Resistant Common Bean Elite Lines
Abstract In Brazil, the Cowpea mild mottle virus vectored by Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is an emerging threat for common bean (Phaseolus vulgaris L.; Fabaceae), including genetically modified elite bean lines developed for resistance to the Bean golden mosaic virus. To investigate this interaction, we quantified the susceptibility of 10 Bean golden mosaic virus-resistant lines to Cowpea mild mottle virus infection in 2 regions in Brazil with natural incidence of viruliferous B. tabaci. In 2 field trials, B. tabaci established on all varieties, but showed preference for the conventional cultivars ‘Pérola’ and ‘BRS Pontal’ when compared with elite lines (averaging > 400 nymphs per leaflet, and > 97% infection rate in 1 study). However, whereas elite lines were resistant to Bean golden mosaic virus, all became infected with Cowpea mild mottle virus. Highest infection rates (19 to 99% infected plants) occurred in genetically modified elite lines derived from BRS Pontal versus Pérola (12 to 16%). When comparing seed yield, most elite lines outperformed their conventional recurrent parent. On average, elite lines achieved relative yield increases between 199 and 1,045%, and the varieties CNFCT 16205 and CNFCT 16210 were the most productive in our field trials. Our results showed that the use of common bean cultivars resistant to golden mosaic is an important tool within integrated management strategies for whiteflies and viruses. However, further studies are needed to elucidate the threat of Cowpea mild mottle virus and the nature of whitefly interactions between these different viruses. Resumo No Brasil, o Cowpea mild mottle virus transmitido pela Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), é uma ameaça emergente ao feijão comum (Phaseolus vulgaris L.; Fabaceae), incluindo as linhas elites de feijão geneticamente modificadas desenvolvidas para resistência ao Bean golden mosaic virus. Para investigar essa interação, quantificamos a suscetibilidade de dez linhas geneticamente modificadas resistentes ao Bean golden mosaic virus à infecção pelo Cowpea mild mottle virus em duas regiões do Brasil com incidência natural de B. tabaci virulíferas. Em dois ensaios de campo, B. tabaci estabeleceu em todas as variedades, mas mostrou preferência pelas cultivares convencionais ‘Pérola’ e ‘BRS Pontal’ quando comparadas com as linhas elite geneticamente modificadas (média > 400 ninfas por folíolo e > 97% taxa de infecção por virus em um dos estudos). No entanto, embora as linhas elite foram resistentes ao Bean golden mosaic virus, todas foram infectadas pelo Cowpea mild mottle virus. As maiores taxas de infecção (19 a 99% de plantas infectadas) ocorreram nas linhas elite geneticamente modificadas derivadas da BRS Pontal versus Pérola (12 a 16%). Ao comparar a produtividade, a maioria das linhas elite superou seu pai convencional recorrente. Os aumentos relativos de rendimento foi de 199 a 1.045% sendo as variedades CNFCT 16205 e CNFCT 16210 as mais produtivas. Nossos resultados mostraram que o uso de cultivares de feijão resistentes ao mosaico dourado é uma ferramenta importante dentro das estratégias integradas de manejo para moscas-brancas e vírus. No entanto, mais estudos são necessários para elucidar à ameaça do Cowpea mild mottle virus e a natureza das interações da mosca-branca com estes diferentes vírus.
期刊介绍:
Florida Entomologist is the official journal of the Florida Entomological Society. Volumes 1-3 were published under the name The Florida Buggist. The Florida Entomological Society still produces the traditionally printed version of Florida Entomologist, but you can also view, search, or print any article published since June 1917 by accessing online files. Web access is made possible by the Society’s electronic publication project begun in 1993