清肝丽水方对微珠致慢性青光眼大鼠视网膜神经节细胞凋亡的神经保护作用

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-04-01 DOI:10.1016/j.jtcm.2018.03.003
Zhang Cheng , Chen Yanqiu , Li Fang , Wang Zhongfeng , Zhang Yinjian
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引用次数: 3

摘要

目的探讨清肝理水方煎剂对微珠致慢性眼内高压(COH)大鼠视网膜神经节细胞(rgc)保护作用的可能机制。方法采用超顺磁氧化铁微珠注入大鼠眼前房制备COH模型。QF在微珠注射后以每天一次6.2 g/kg的剂量灌胃给药,直至第28天。采用霍乱毒素B亚单位(CTB)逆行标记和免疫组化评价视网膜RGCs数量的变化。采用末端dUTP缺口末端标记(TUNEL)染色评价rgc细胞凋亡变化。结果注射微珠可使大鼠眼压稳定升高。微珠注射后2-4周,IOP升高导致ctb标记的rgc数量逐渐减少。QF可适度降低COH模型大鼠IOP,减弱COH大鼠ctb标记RGCs数量的减少。此外,微珠注射后2-4周,IOP升高导致tunel阳性RGC数量逐渐增加,提示RGC凋亡程度增加。与未治疗的COH视网膜相比,qf治疗的COH视网膜中tunel阳性信号的数量显著减少。结论芪黄汤对COH视网膜的RGC具有保护作用,其机制可能是减少RGC的损失;这些作用可能是通过抑制RGC细胞凋亡介导的。
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Neuroprotective effect of Qinggan Lishui formula on retinal ganglion cell apoptosis in a microbead-induced rat chronic glaucoma model

OBJECTIVE

To investigate a possible mechanism for protective effects of a decoction of the Qinggan Lishui formula (QF) on retinal ganglion cells (RGCs) in a rat model of microbead-induced chronic intraocular hypertension (COH).

METHODS

The COH model was generated by injecting microbeads (superparamagnetic iron oxide) into the anterior chamber of rat eyes. QF was given by intragastric administration (gavage) once daily at a dose of 6.2 g/kg until day 28, following microbead injection. Cholera toxin B subunit (CTB) retrograde labeling and immunohistochemistry were used to evaluate changes in the number of RGCs in the retina. Terminal dUTP nick end labeling (TUNEL) staining was used to assess apoptotic changes in RGCs.

RESULTS

Microbead injection induced a steady increase in intraocular pressure (IOP) of rats. Elevated IOP resulted in a progressive reduction in the number of CTB-labeled RGCs, 2-4 weeks after microbead injection. QF administration may moderately reduce IOP in the rat COH model and attenuate reduction of the number of CTB-labeled RGCs in COH rats. Furthermore, elevated IOP resulted in a progressive increase in the number of TUNEL-positive RGCs, 2–4 weeks after microbead injection, suggestive of an increase in the extent of RGC apoptosis. There was a significant reduction in the number of TUNEL-positive signals in QF-treated COH retinas, compared with untreated COH retinas.

CONCLUSION

QF decoction may provide a protective effect for RGCs in COH retinas by reducing RGC loss; these effects may be mediated by inhibition of RGC apoptosis.

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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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