Potato (Solanum tuberosum L.) Plantlet Regeneration in Ammonium Nitrate Free Stock Solution-1 of Murashige & Skoog (MS, 1962) Plant Tissue Culture Medium

Potato (Solanum tuberosum L.) plantlet regeneration potentiality was studied in ammonium nitrate (NH4NO3) free tissue culture medium. Four different treatments were designed from the compositions of the stock solution-01 of plant tissue culture medium. Those were -Treatment-1 (Stock solution -01 as recommended by Murashige & Skoog (1962), Treatment-2 (MS stock solution-01 without having NH4NO3), Treatment-3 (MS stock solution-01without NH4NO3 but other component had double concentration), Treatment-4 (Readymade MS powder, Duchefa Biocheme, The Netherland). Shoot length, shoot diameter, node number and leaf number per plantlet were highest in Treatment-4 at 14, 21 and 28 days after inoculation (DAI). Shoot regeneration parameters were statistically similar with the treatment-3 and the check treatment-1. But the check treatment-1 showed better result in root number and root length (cm) as compared to treatment-3 and treatment-4. The treatment-2 showed lowest result in each of the said parameter. The stock solution-01 which was formulated without ammonium nitrate and has double dose of other ingredient has the potentiality for potato plantlet regeneration, but it was not as suitable as Readymade MS powder (Duchafa, The Netherland).